HRX-EEN融合基因转基因小鼠行为能力的异常  

Abnormality on behavioral ability of transgenic mice for HRX-EEN fusion gene

作　　者：蔡军[1] 傅爱芬[1] 郑林[1] 傅国辉[1] 

机构地区：[1]上海交通大学基础医学院病理学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第1期31-33,共3页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海交通大学医学院科技基金(06XJ21012)~~

摘　　要：目的观察HRX-EEN融合基因转基因小鼠行为能力的异常。方法应用Morris水迷宫实验测试并比较HRX-EEN融合基因转基因小鼠(转基因组,n=12)和C57/BL小鼠(对照组,n=12)的学习和记忆能力,包括前4天的隐蔽平台实验和第5天的空间探索实验。结果隐蔽平台实验中,转基因组小鼠的逃避潜伏期长于对照组,除第1天外,其余各天差异均具有统计学意义(P<0.05);空间探索实验中,转基因组小鼠目标象限内搜索时间、经过目标平台位置次数和在中心区域时间显著少于对照组(P<0.01)。结论HRX-EEN融合基因转基因小鼠的学习和记忆能力显著下降。Objective To observe the abnormality on behavioral ability of transgenic mice for HRX-EEN fusion gene. Methods Transgenic mice for HRX-EEN fusion gene （transgenic group, n = 12） and C57/BL mice （ control group, n = 12 ） were tested in hidden platform training （ day 1 to day 4） and probe trial testing （ day 5） in Morris water maze in which ability of spatial learning and retention was assessed. Results In hidden platform training, the latencies of transgenic group were longer than those in control group, and significant differences were observed between the two groups for day 2, 3 and 4 （ P 〈 0.05）. In probe trial testing, the searching time in target quadrant, the times of crossing previous platform and the time in central area of transgenic group were significantly less than those of control group （ P 〈 0.01 ）. Conclusion The ability of spatial learning and retention is significantly decreased in transgenic mice for HRX-EEN fusion gene.

关 键 词：HRX-EEN融合基因 转基因小鼠 MORRIS水迷宫 

分 类 号：Q78[生物学—分子生物学]