急性川崎病外周血单个核细胞核因子-κB活化的变化  

Nuclear Factor-κB Activation in Peripheral Blood Mononuclear Cells during Acute Kawasaki Disease

作　　者：邹峥[1] 熊国亮[2] 段君凯[3] 刘珍琼[2] 许飞[3] 卢巧[1] 

机构地区：[1]江西省儿童医院风湿免疫科,南昌330006 [2]江西省胸科医院检验科,南昌330008 [3]江西省儿童医院心脏病中心,南昌330006

出　　处：《实用儿科临床杂志》2009年第1期35-37,共3页Journal of Applied Clinical Pediatrics

基　　金：江西省科技厅社会发展攻关项目(医疗卫生科技研究)资助

摘　　要：目的探讨外周血单个核细胞(PBMC)中核因子-κB(NF-κB)活化在急性川崎病(KD)发生、发展中的作用。方法分别从急性期KD患儿30例及健康儿童20例的外周血中分离出PBMC,并分别分为3组培养,即自然培养下的空白对照组、加入蛋白激酶C(PKC)激活剂佛波醇乙酯(PMA)的PMA组和同时加入PMA及NF-κB抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)的PMA加PDTC组,采用免疫组织化学染色法检测各组PBMC中NF-κB活化率。结果在自然培养下,KD空白对照组的NF-κB活化显著高于健康空白对照组(P<0.01);KDPMA组的NF-κB活化率显著高于KD空白对照组和健康对照PMA组(Pa<0.01),而KDPMA加PDTC组的NF-κB活化率显著低于急性期KDPMA组(P<0.01)。自然培养情况下,急性期KD患儿有冠状动脉损害(CAL)亚组的NF-κB活化率显著高于KD无CAL亚组(P<0.01);在PMA刺激后,KD有CAL亚组NF-κB活化率亦显著高于KD无CAL亚组(P<0.01)。KD患儿急性期NF-κB活化率与血清CRP及外周血WBC计数均无相关性(Pa>0.05)。结论NF-κB活化参与急性期KD血管炎的发生和CAL的形成。急性期KD患儿PBMCs中NF-κB活化受PKC信号传导通路调控,PDTC显著抑制NF-κB活化。Objective To explore the significance of nuclear factor - KB （ NF - KB） activation in peripheral blood mononuelear cells （PBMC） during acute Kawasaki disease（KD）. Methods Peripheral blood was collected from children with acute KD （n = 30） and healthy age -matched children （n = 20）. PBMC were cultured in vitro and divided into 3 groups：naturally cultured blank control group, protein ki- nase C （PKC） activator stimulated phorbol 12 - myristate 13 - acetate （PMA） group and PMA plus NF - KB inhibitor treated PMA plus pyr- rolidine dithiocarbamate （PDTC）group. Percentages of NF - KB activation were detected by immunohistochemistry. Results Under natural culturing,the percentage of cells with activated NF - KB was significantly higher in acute KD blank control group than that in healthy blank control group. The percentage of cells with activated NF - KB was significantly higher in acute KD PMA group than that in acute KD blank group and that in normal control PMA group, respectively （ P 〈 O. O1 ）. The percentage of cells with activated NF - KB was significantly lower in acute KD PMA plus PDTC group than that in acute KD PMA group （ P 〈 0.01 ）. Under natural culturing, the percentage of cells with activa- ted NF - KB in acute KD with coronary artery lesions （CAL） was higher than that in without CAL（P 〈 0.01 ）. After PMA stimulation, the a- bove percentage was further higher in acute KD with CAL than that in the disease without CAL （ P 〈 O. O1 ）. There was no correlation between the percentage of NF - KB activation with either serum CRP level or WBC count level in peripheral blood of children with acute KD （ Po 〉 0.05）. Conclusions NF - KB activation in PBMC during acute KD is markedly increased, which suggests that NF - KB activation plays an important role in the formation of vasulitis and CAL in this disease. NF - KB activation in PBMCs in children with KD is regulated by the PKC signaling pathway and PDTC obviously inhibits the activation of NF - KB.

关 键 词：核因子-KB 核因子-KB抑制剂 皮肤黏膜淋巴结综合征 

分 类 号：R725.4[医药卫生—儿科]