肺出血新生大鼠肺组织前列环素和血栓素B_2的变化  

Changes of Prostacyclin and Thromboxane B_2 in Lung Tissue of Newborn Rats with Pulmonary Hemorrhage

作　　者：吴春英[1] 游上游[1] 鲁建生[1] 胡红兵[1] 康世秀[1] 周泉[1] 孙金枝[1] 

机构地区：[1]武汉市儿童医院新生儿科,武汉430016

出　　处：《实用儿科临床杂志》2009年第2期124-125,139,共3页Journal of Applied Clinical Pediatrics

基　　金：武汉市卫生局临床重点学科研究项目资助(2004-241)

摘　　要：目的观察肺出血新生大鼠肺组织6-酮-前列腺素(6-k-PG)、血栓素B2(TXB2)的动态变化,探讨新生儿肺出血的发病机制。方法新生大鼠40只,随机分为实验1组、实验2组、实验3组及正常对照组,每组10只。实验1、2、3组大鼠背部皮下注射1g/L肾上腺素注射液0.5mL/kg后放4℃冷室。实验1组1h后拿出,断头处死;实验2组4h拿出,断头处死;实验3组4h拿出后复温,同时注射肾上腺素1次,剂量不变,间隔1h再注射1次,剂量加倍,出冷室后2h断头处死。肉眼观察其肺组织大体病理情况,将结果分为5级:Ⅰ级正常肺,Ⅱ级肺水肿,Ⅲ级点状肺出血,Ⅳ级局灶性肺出血,Ⅴ级弥散性肺出血。采用放射免疫法测定其肺组织6-k-PG和TXB2水平。结果1.肺大体病理改变:实验1组Ⅱ级肺9只、Ⅴ级肺1只;实验2组Ⅱ级肺6只、Ⅴ级肺4只;实验3组Ⅲ级肺4只、Ⅳ级肺1只、Ⅴ级肺5只;正常对照组大鼠Ⅰ级肺10只,组间比较差异有极显著意义(P<0.01)。Ⅴ级肺电镜下见肺泡腔内有出血,部分气血屏障结构模糊。2.实验1组肺组织TXB2水平与实验2组、实验3组及正常对照组比较均明显下降(Pa<0.05)。实验1组6-k-PG/TXB2值与实验2组、实验3组及正常对照组比较均明显增高(Pa<0.05)。结论在新生大鼠肺出血的形成过程中,前列环素(PGI2)作用相应增加,PGI2使血管通透性增加,参与肺出血的发生。Objective To observe the changes of thromboxane B2（TXB2）and 6 -keto - prostaglandin （6 -k -PG ）in the lungs of the newborn rats with pulmonary hemorrhage and explore the pathogenesis of pulmonary hemorrhage in newborn infants. Methods Forty newborn rats were divided randomly into 4 groups ：3 experimental groups（ experiment 1 group, experiment 2 group, experiment 3 group） and a normal control group （n = 10 each group）. The rats in experiment 1 group, experiment 2 group, experiment 3 group were given subcutaneous injection of 1 g/L epinephrine at a dose of 0.5 mL/kg and were put into a refrigerator of 4 ℃. The rats in experiment 1 group were taken out of the refrigerator after 1 h and were killed. The rats in experiment 2 group and experiment 3 group were taken out after 4 h. The rats in experiment 2 group were killed. The rats in experiment 3 group were rewarmed and were given epinephrine at an equal dose and were given epinephrine again after 1 h at a dose of 1.0 mL/kg, after 1 h, they were killed. Gross anatomical findings of the lungs were divided into 5 grades ： grade Ⅰ（ norreal lung）, grade Ⅱ （ pulmonary edema）, grade Ⅲ （ spotty pulmonary hemorrhage ）, grade Ⅳ （ local pulmonary hemorrhage ） and grade Ⅴ （ diffuse pulmonary hemorrhage）. TXB2 and 6 - k - PG in the lungs were nleasured by radioimmunoassay. Results 1. The pathologic changes of the lungs were revealed in 9 cases of grade Ⅱ and 1 case of grade Ⅴ in experiment 1 group;6 cases of grade Ⅱ and 4 cases of grade Ⅴ in experiment 2 group;4 cases of grade Ⅲ,1 case of grade Ⅳ and 5 cases of grade V in expriment 3 group;10 cases of grade Ⅰ in coutrol group. By the RIDIT analysis significant difference was found（P 〈 0.01 ）. Electron microscopy exam showed erythrocytes in the alveoli and blurred structure of part blood - gas barrier for grad V. 2. The level of TXB2 was significantly lower in experiment 1 group than that in experimnet 2 group ,experimnet 3 group and control group（Pa 〈0.05

关 键 词：血栓素B2 前列环素 肺疾病 出血 大鼠 新生 

分 类 号：R722.1[医药卫生—儿科]