反复高热与高热惊厥对幼年大鼠脑区生长抑素水平的影响及其意义  

Effects of Repeated Hyperthermia and Febrile Seizure on Somatostatin Content in Brain of Rats and Its Signi-ficance

作　　者：闫秀梅[1] 李光乾[1] 

机构地区：[1]温州医学院附属育英儿童医院神经内科,浙江温州325027

出　　处：《实用儿科临床杂志》2009年第2期137-139,共3页Journal of Applied Clinical Pediatrics

基　　金：温州市科技局科技发展计划项目(Y2004A012)

摘　　要：目的探讨反复高热和高热惊厥(FS)对幼年大鼠脑区生长抑素(SS)水平的影响。方法雄性SD大鼠51只。随机分为正常对照组(NC组,n=14只)、高热对照组(HC组,n=19只)和FS组(n=18只)。水浴法建立FS模型。免疫组织化学法检测各组大鼠海马齿状回(DG)、CA1区、CA3区以及颞叶皮层(CTL)中SS的表达情况,放射免疫法检测各组大鼠海马匀浆中SS水平。结果免疫组织化学结果显示HC组大鼠脑组织SS阳性细胞数DG区(21.10±2.51)、CA3区(10.60±2.41)较NC组(15.50±3.20,8.30±1.49)明显增加,FS组DG区(10.50±2.12)、CA3区(6.90±2.02)较NC组减少,各组CA1区与CTL均未发生明显变化;放射免疫结果显示,各组大鼠海马匀浆中SS水平两两比较,HC组[(53.74±7.56)ng/g]明显高于NC组[(35.70±2.90)ng/g](P<0.01)和FS组[(39.54±7.74)ng/g](P<0.05),FS组与NC组比较无显著性差别(P>0.05)。结论反复高热和FS后,幼年大鼠脑组织各区SS发生不同的变化,提示SS可能有增加惊厥易感性和促进惊厥的作用。Objective To explore the change of somatostatin（SS） in brain areas of juvenil rats with repeated febrile seizures （FSs） and hyparthermia. Methods Fifty - one male SD rats were divided randomly into 3 groups ： normal group （ NC group, n = 14 ）, hyperthermic group （ HC group, n = 19） and febrile seizure group （ FS group, n = 18 ）. FS were induced by placing rats in a bath of water. The expressions of SS in DG, CA3, CA1 and CTL were measured by immunohistochemistry. The level of SS in hippocamps of these rats were determined by radioimmunoassay. Results The results of immunohistochemistry shew in HC group,the number of the SS positive neuron were increased in DG（21.10 ± 2. 51 ）, CA3 （ 10.60 ± 2.41 ） in FS group, which was less than that in NC group （ 10.50 ± 2.12,6.90 ± 2.02 ）, there was no difference in CA1 and CTL. The result of Radioimmunoassay ：the level of SS in hippocampus in HC group [ （ 53.74 ± 7.56 ） ng/g ] was higher than that in FS group [ （39.54 ± 7.74 ） ng/g ] （ P 〈 0.05 ） and NC group [ （ 35.70 ± 2.90 ） ng/g ] （ P 〈 0. 01 ）, but there was no significantly difference between FS group and NC group （P 〉 0.05 ）. Conclusion There is different change of somatostatin content in some brain areas of rats with repeated febrile seizure, which suggest that SS can increase the affeetivity of seizure and promote the seizure.

关 键 词：高热惊厥 生长抑素 海马 大鼠 

分 类 号：R720.597[医药卫生—急诊医学]