川芎嗪衍生物对人阴茎海绵体平滑肌细胞游离钙浓度的影响  

Effect of Ligustrazine Derivative on Cytosolic Free Calcium Concentration in Smooth Muscle Cells from Human Spongy Body of Penis

作　　者：张立冬[1] 刘继红[2] 李邃 刘妙娜[1] 林智成[1] 严书超[1] 尹春萍[1] 

机构地区：[1]华中科技大学同济医学院药学院,武汉430030 [2]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [3]贵州省六盘水市药品检验所,553001

出　　处：《医药导报》2009年第2期148-150,共3页Herald of Medicine

基　　金：湖北省科技攻关计划资助项目(基金编号:2007AA301B26-1)

摘　　要：目的研究川芎嗪(TMP)衍生物(A3-A6)对人阴茎海绵体平滑肌细胞(PCSMC)胞质内游离钙离子(Ca2+)浓度的影响。方法采用新型Ca2+荧光染色剂Fluo-3/AM负载人PCSMC,应用激光扫描共聚焦显微镜(LSCM)实时测定胞质内[Ca2+]i变化。以母体川芎嗪和经典钙拮抗药维拉帕米(Ver)为阳性对照,分别观察A3-A6对去甲肾上腺素(NE)诱导胞质内钙浓度升高的影响。结果在浓度0.2 mmol.L-1时,A3-A6对NE诱发的人PCSMC内[Ca2+]i升高有明显抑制作用,抑制率分别为49.03%,54.83%,51.48%和50.31%,优于TMP(18.96%),远大于Ver(16.51%)(P<0.05)。结论A3-A6对人PCSMC电压依赖性钙通道均有抑制作用,能降低PCSMC胞质内Ca2+水平,其作用效果均比TMP强。Objective To study the effects of ligustrazine derivatives （A3-A6） on intracellular free calcium （ Ca^2＋ ）i in cultured human penis corpus cavernosum smooth muscle cells （PCSMC）. Methods By using laser scanning confocal microscope（LSCM） ,the Ca^2＋ Fluorescence signal changes was investigated in cultured PCSMC loaded with Ca^2＋ indicator Fluo- 3/AM. Compared with TMP and the classical calcium antagonist Ver, effects of ligustrazine derivatives on （ Ca^2＋ ） i increase induced by NE were observed at the same concentration. Results The peak inhibition rates of ligustrazine derivatives （ A3- A6） were 49.03%, 54.83% ,51.48% ,50.31%, respectively induced by NE at the concentration of 0.2 mmol· L^-1, which were more potent than ligustrazine （ 18.96% ） , much more than Ver （ 16.51% ） （ P 〈 0.05 ）. Conclusion Ligustrazine derivatives （A3-A6） can inhibit Man PCSMC （Ca^2＋ ）i , significantly by suppressing voltage dependent calcium channel, which is more effective than TMP.

关 键 词：川芎嗪衍生物 平滑肌细胞 激光扫描共聚焦显微镜 钙通道 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]