通心络胶囊对大鼠局灶性脑梗死后微血管新生的影响  

Effects of Tongxinluo on VEGF Expression and Angiogenesis of Rats with Cerebral Infarction

作　　者：杨业新[1] 叶连珍[1] 何刚[1] 梁新宇 梅元武[2] 

机构地区：[1]中国地质大学(武汉)医院,430030 [2]华中科技大学同济医学院附属协和医院神经内科,武汉430022

出　　处：《医药导报》2009年第2期182-184,共3页Herald of Medicine

摘　　要：目的探讨通心络对局灶性脑缺血后微血管新生的影响。方法将55只SD大鼠随机分为假手术对照组5只和模型组、通心络组各25只;假手术对照组给予假手术,模型组和通心络组以线栓法制作左侧大脑中动脉栓塞脑梗死模型,通心络组术后1 d给予通心络1 g.kg-1.d-1灌胃,共14 d;其余两组常规饲养。以免疫组织化学法检测血管内皮生长因子(VEGF)和血浆Ⅷ因子相关抗原(vWF),测定微血管密度(MVD)。结果大鼠大脑中动脉栓塞后,缺血区神经元变性、坏死,VEGF和vWF在缺血边缘区有少量表达,经通心络干预后,VEGF和vWF大量表达,微血管数目明显增加。结论通心络可通过促VEGF表达上调促进微血管新生。Objective To evaluate the effects of tongxinluo on VEGF expression and angiogenesis and functional outcome after cerebral infarction in rats. Methods 55 male SD rats were divided into control group, model group and tongxinluo （TXL） group randomly. The rat model of focal cerebral infarction was established with unilateral middle cerebral artery （MCA） suture occlusion in the model group and TXL group. The rats in TXL group were given by gavage with TXL （1g·kg^-1·d^-1 ） for 14 d, while those in other groups were reared in their original living state. VEGF expression and angiogenesis were measured in the boundary zone to brain infarction at 1, 3, 7, 14 and 28 d. Results It was found that VEGF expression started at 1 d, subsequently increased, and reached peak value at the 3 d, failed away until 7 d in model group and TXL group. VEGF expression was mainly detected in the boundary zone of the infarcts at every timepoint. To compare with the model group, VEGF expression of TXL group was significantly increased in 3 and 7 d after MCAO. Compared with the control group, the microvessel density （MVD） in model group and TXL group were significantly increased at 14 and 28 d after MCAO, especially in TXL group. Conclusion Tongxinluo may increase the VEGF expression and promote the blood vessel proliferation in rats with cerebral infarction.

关 键 词：通心络 脑缺血 血管内皮生长因子 毛细血管新生 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]