磺丁基醚-β-环糊精手性流动相添加剂法分离兰索拉唑对映体  

Resolution of lansoprazole enantiomers using sulfobutylether-beta-cyclodextrin as a chiral additive by high performance liquid chromatography

作　　者：关瑾[1,2] 杨晶[2] 石爽[1] 阎峰[1] 李发美[2] 

机构地区：[1]沈阳化工学院应用化学学院,沈阳110142 [2]沈阳药科大学药学院,沈阳110016

出　　处：《分析试验室》2009年第2期91-93,共3页Chinese Journal of Analysis Laboratory

基　　金：沈阳市科技局(1063307-1-03)项目资助

摘　　要：采用磺丁基醚-β-环糊精(SBE-β-CD)为手性流动相添加剂,建立了兰索拉唑对映体的高效液相色谱分离分析方法。对影响兰索拉唑对映体分离的主要因素:环糊精种类和浓度、缓冲溶液pH以及有机改性剂种类和含量进行考察。确定最优色谱条件:色谱柱为Spherigel C18(150mm×4.6mm,5μm),流动相为V(乙腈)∶V(水相)=20∶80(水相含10mmol/L SBE-β-CD、10mmol/L NaH2PO4缓冲液、pH2.5),流速为0.9mL/min,检测波长为288nm。在此条件下,兰索拉唑对映体的保留时间分别为14.4和15.8min,分离度为2.0。两对映体质量浓度在0.2～50μg/mL范围内线性关系良好(r≥0.9996),保留时间的RSD分别为0.27%和0.26%,峰面积的RSD分别为0.65%和0.68%。A new high performance liquid chromatographic （HPLC） method using sulfobutylether-beta-cyclodextrin （SBE-β-CD） as a chiral mobile phase additive （CMPA） was developed for the enantioseparation of lansoprazole. The influences of type and concentration of CD, acetonitrile content and buffer pH of mobile phase on the resolution and retention of enantiomers were investigated. A baseline resolution （Rs = 2.0） of lansoprazole enantiomers was achieved on a Spherigel Cls column （150 mmn× 4.6 mm, 5μm） using acetonitrile： aqueous phase = 20：80 （aqueous phase was composed of 10 mmol/L SBE-β-CD, 10 mmol/L phosphate buffer, pH 2.5） as the mobile phase with a flow rate of 0.9 mL/min at room temperature, and UV detection at 288 nm. The retention times of lansoprazole enantiomers were 14.4 min and 15.8 min, respectively. A good linearity was observed for each enantiomer in the concentration range of 0.2 - 50 μg/mL with r≥0.9996. The RSDs for the retention time were 0.27% and 0.26%, respectively. The RSDs for peak area were 0. 65 % and 0.68 %, respectively. The method is simple, economic and the reproducibility is fine.

关 键 词：手性流动相添加剂 高效液相色谱法 兰索拉唑对映体 磺丁基醚-Β-环糊精 手性分离 

分 类 号：O552.6[理学—热学与物质分子运动论]