生物瓣膜材料体外内皮化形态学观察方法的探讨  

A Method of Morphological Observation on the Endothelialization of Bioprosthetic Heart Valve Materials in Vitro

作　　者：魏蜀亮[1] 石应康[2] 王树人[3] 

机构地区：[1]川北医学院附院胸外科,南充63700 [2]华西医科大学附一院胸外科,成都610041 [3]华西医科大学基础医学院病理生理教研室,成都610041

出　　处：《生物医学工程学杂志》1998年第2期148-150,162,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金

摘　　要：在牦牛心包生物心瓣膜材料体外内皮化实验中，采用瑞氏染色法，成功地在普通光镜下观察到了心包片上种植的血管内皮细胞形态、多少及排列状况。与扫描电镜（ＳＥＭ）相比，该方法简便易行，时效性强，可随时进行，为实验室进行生物材料内皮化的形态学观察提供了方便，具有推广价值。In order to examine the endothelialization of yak pericardial materials in vitro,we used Wright staining and successfully observed the morphology,number and order of endothelial cells seeded on pericardial patches under light microscope.Compared with scanning electron microscopy,the method of light microscopy is simple,easy to do and may be carried out at any time.In laboratory,it brings much convenience in morphological observation on the endothelialization of biomaterials and therefore is worth recommendation.

关 键 词：生物材料 内皮化 形态学 心瓣膜 

分 类 号：R318.11[医药卫生—生物医学工程] R318.08[医药卫生—基础医学]