脾阴虚证大鼠海马蛋白质组双向凝胶电泳的研究  

Studies on hippocampus proteome of rats with syndrome of spleen yin deficiency by two-dimensional gel electrophoresis

作　　者：战丽彬[1] 刘莉[2] 路小光[2] 宫晓洋[3] 

机构地区：[1]大连医科大学附属二院中医科,大连116023 [2]大连大学附属中山医院急诊科,大连116001 [3]大连医科大学附属一院中医科,大连116011

出　　处：《中华中医药杂志》2009年第2期149-152,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30472255)

摘　　要：目的:应用双向凝胶电泳方法初步分析脾阴虚证大鼠海马蛋白质组的表达,为进一步探讨脾阴虚所致脑损伤的分子机制提供依据。方法:采用饮食不节、劳倦过度联合耗伤阴液法建立脾阴虚证大鼠模型,对脾阴虚组和空白对照组海马蛋白质组表达进行比较,观察分析其差异。结果:脾阴虚组出现易激惹、咬斗,肛温升高,饮水量增加等脾阴虚表现(P<0.05)。通过双向凝胶电泳图谱比较发现脾阴虚组海马蛋白质组中有6个蛋白点表达发生明显改变,其中5个蛋白点表达明显下调,1个表达显著上调。结论:应用双向凝胶电泳方法初步筛选出脾阴虚大鼠海马蛋白质组的差异表达,推测与学习记忆相关。Objective： To apply two dimensional gel electrophoresis （2-DE） to initially analyze the protein expression in hippocampus of rats with spleen yin deficiency syndrome and to get the theory foundation for investigating the molecular mechanisms of Alzheimer＇s Disease and spleen yin deficiency syndrome. Methods： Rats model of spleen yin deficiency were set up by combination of improper diet, overstrain and consumption of body fluid.Qualitative changes of the proteome expression in hippocampus were compared between the model group and control group by two-dimensional gel electrophoresis. Results： The model group of spleen yin deficiency had the appearance of spleen yin deficiency, for example, irritability, increment in biting frequency, increase of rectal temperature and drinking water （P〈0.05）. There were obvious differences in expression of six protein spots between the model group and control group. Five protein spots showed lower expression and one spot increased significantly. Conclusions： The changes discovered by 2-DE in expression level and modification of some proteins may contribute to learning and memory abilities.

关 键 词：脾阴虚 海马 学习记忆 蛋白质组 双向凝胶电泳 

分 类 号：R256.3[医药卫生—中医内科学]