抗纤益心方对扩张型心肌病大鼠心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响  

Effect of Kangxianyixin prescription on cardiocyte apoptosis and expression of Bax and Bcl-2 in rRats with dilated cardiomyopathy

作　　者：王振涛[1] 韩丽华[1] 朱明军[2] 曹程浩[1] 柴松波[1] 

机构地区：[1]河南省中医院心血管病研究室,郑州450011 [2]河南中医学院第一附属医院心脏中心,郑州450003

出　　处：《中华中医药杂志》2009年第2期152-155,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省高校新世纪优秀人才支持计划(No.2005HANCET-07)

摘　　要：目的:观察抗纤益心方对扩张型心肌病大鼠心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响。方法:喂饲呋喃唑酮建立扩张型心肌病动物模型,随机分组后药物干预8周,检测心肌凋亡及心肌Bcl-2、Bax蛋白表达。结果:①中西药联用组、抗纤益心方大剂量组、卡托普利组、抗纤益心方小剂量组心肌细胞凋亡指数与模型组比较有显著差异(P<0.05),其中以中西药联用组减少凋亡最明显;②与模型组相比,中西药联用组、大剂量组和卡托普利组均能明显下调Bax蛋白表达(P<0.05),上调Bcl-2蛋白表达(P<0.05)。结论:抗纤益心方有抑制或减少扩张型心肌病大鼠心肌细胞凋亡的作用,其机制与下调Bax蛋白表达、上调Bcl-2蛋白表达有关。Objective：To expound the effect of Kangxianyixin prescription on cardiocyte apoptosis and expression of Bax and Bcl-2 in rats with dilated cardiomyopathy. Methods： Wistar rats with dilated cardiomyopathy established by furazolidone were treated 8 weeks with Kangxianyixin prescription, to observe cardiocyte apoptosis and to measure Bax and Bcl-2 expression in rats, Results： （1） In comparison of cardiomyocyte apoptosis indexes in rats, when compared with model group, the results showed significant difference in the combined group of high dosage of Kangxianyixin prescription and captopril, the high dosage group, the low dosage group, the captopril group （P〈0.05） ; （2） Compared with the model group, the combined group of high dosage of Kangxianyixiu prescription and captopril, the high dosage group and the captopril group could dramatically lower Bax gene expression （P〈0.05）and increase Bcl-2 gene expression（P〈0.05）. Conclusion： Kangxianyixin prescription can restrain or decrease cardiomyocyte apoptosis, it may be interrelated with reducing Bax expression and increasing Bcl-2 expression.

关 键 词：抗纤益心方 扩张型心肌病 心肌细胞凋亡 实验研究 

分 类 号：R285.5[医药卫生—中药学]