雄性小鼠肾虚对其雄性仔鼠睾丸组织氧化应激水平的影响  

Effect of Kidney-jing Deficiency on oxidative stress of male offspring

作　　者：孙洁[1] 周安方[2] 周艳艳[2] 方婷[2] 

机构地区：[1]浙江省中医院,杭州310006 [2]湖北中医学院,武汉430061

出　　处：《中华中医药杂志》2009年第2期208-210,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖北省自然科学基金资助课题(No.2004ABA184)

摘　　要：目的:观察雄性小鼠肾虚对其雄性仔鼠睾丸氧化应激水平的影响。方法:将30只雄性昆明小鼠用计算机取随机数法随机分为正常组、模型组和补肾组3组。其中模型组和补肾组采用房劳加惊恐的复合伤肾法制造肾精亏虚模型。补肾组每天给予0.16ml/10g体质量的补肾填精方浓缩液灌胃。正常组及模型组予等量生理盐水灌胃,造模并给药21d。从造模结束次日起,所有小鼠与正常发情雌鼠配对饲养5d后,取出雌鼠待其分娩。仔鼠饲养至6周龄时每组随机取出10只雄性仔鼠,取出左侧睾丸,检测其组织匀浆上清中丙二醛(malondialdehyde,MDA)、过氧化物歧化酶(superoxide dismutase,SOD)活性、总抗氧化能力(total antioxygen capacity),T-AOC。结果:模型组仔鼠睾丸组织MDA水平(24.26±6.05)μmol/gprot明显高于正常组(16.54±1.47)μmol/gprot及补肾组(15.04±1.50)μmol/gprot,(P<0.01)。模型组仔鼠睾丸组织T-AOC水平(2.71±0.55)U/mgprot与正常组仔鼠(4.33±1.54)U/mgprot比较明显降低(P<0.01),与补肾组(4.08±0.91)U/mgprot比较亦有所下降(P>0.05)。补肾组与正常组之间无显著性差异(P>0.05)。而各组仔鼠睾丸组织SOD水平之间均无显著性差异(P>0.05)。结论:模型组仔鼠睾丸内抗氧化能力下降,并可能因此而导致氧化应激水平上升,补肾法可以有效地拮抗这种病理变化。睾丸内氧化抗氧化体系的失衡可能是肾虚仔鼠生育力下降的重要原因之一。Objective： To investigate the effects of kidney-jing deficiency of male mice on the oxidative stress of their male offspring. Methods： Thirty 6-weeks-old Kunming male mice were divided into three groups randomly： the blank control group, the model group and the kidney-tonifying group. The model and the kidney-tonifying groups were stressesd by fear plus excessive sex to establish a kidney-jing deficiency model, and meanwhile the latter were given concentrated solution of Kidneytonifying recipe intragastrically at the does of 0.16ml/10g. The control and the model groups were treated with physicological saline at the same dose for 21 days. Then all the male mice were mated with the healthy estrous females for 5 days. The malondialdehyde（MDA）, superoxide dismutase （SOD） and total antioxygen capacity （T-AOC） of testicle of male offsprings were detected at 6 weeks. Results： The level of MDA in model group was higher than that in blank control group and kidney-tonifying group significantly（（24.26±6.05） vs（16.54±1.47）,（15.04±1.50）μmol/gprot（P〈0.01）, and the level of T-AOC in model group were lower than that in normal group and kidney-tonifying group significantly（2.71±0.55）of model group vs（4.33±1.54）U/mgprot of blank group, P〈0.01, （2.71±0.55）of model group vs（4.33±1.54）U/ingprot of Kidney-tonifying group（P〈0.05）. There were no statistical difference of the level of SOD between groups. Conclusion： The fear plus excessive sex could reduce AOC level of male offspring, and kidney-tonifying could rivalry this pathological change. The results means the variation of oxidative system was one of the reasons for fertility damage on these male offsprings.

关 键 词：肾精亏虚 动物模型 子代 补肾填精方 氧化应激 

分 类 号：R256.5[医药卫生—中医内科学]