人绒毛膜促性腺激素对3AO细胞bcl-2和bax表达的影响  

Effect of HCG on bcl-2 and bax expressions of ovarian caner 3AO cells

作　　者：邢艳[1] 杨明辉[1] 游艺[2] 赖明唏[2] 唐中[2] 

机构地区：[1]川北医学院附属医院风湿免疫研究所,四川南充637000 [2]川北医学院附属医院检验科,四川南充637000

出　　处：《检验医学与临床》2009年第3期161-162,共2页Laboratory Medicine and Clinic

摘　　要：目的初步分析人绒毛膜促性腺激素(HCG)对3AO细胞内凋亡相关基因bcl-2和bax在蛋白质和mRNA水平表达的影响,探讨HCG信号在癌细胞增殖凋亡中的作用机制。方法用不同浓度HCG处理卵巢癌3AO细胞72h,采用流式细胞术间接免疫荧光法和实时定量逆转录聚合酶链式反应法分别测定3AO细胞内凋亡蛋白质bcl-2和bax在蛋白质和基因水平的表达。结果HCG不改变3AO细胞bcl-2、bax的表达(P>0.05)。结论HCG在体外抑制卵巢癌细胞凋亡的作用可能与凋亡基因bcl-2和bax无关。Objective To preliminarily assay the effect of human chorionic gonadotrophin （HCG） on the protein and mRNA expressions of bcl 2 and bax in ovarian caner 3AO cells,and to explore the mechanism of HCG signal in proliferation and apoptosis of ovarian cancer cells. Methods The ovarian caner 3AO ceils were treated for 72 hours with different concentrations of HCG. The protein and mRNA expressions of bcl-2 and bax of 3AO cells were detected by flow cytometrie indirect immunofluorescent technique and real time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results HCG did not change the expressions of bcl-2 and bax in ovarian carcinoma 3AO cells. Conclusion The inhibitory action of HCG on apoptosis of ovarian carcinoma 3AO cells may not correlate with apoptosis gene bcl-2 and bax.

关 键 词：卵巢肿瘤 凋亡 人绒毛膜促性腺激素 流式细胞术 

分 类 号：R373[医药卫生—病原生物学]