微卫星标记BM3413和OB2在5个绵羊群体中多态性的研究  

Polymorphism of BM3413 and OB2 Microsatellites DNA in 5 Sheep Breeds

作　　者：张雨[1] 张建新[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《畜禽业》2009年第2期40-43,共4页Livestock and Poultry Industry

基　　金：山西省青年科学基金(项目编号2006021037);山西省攻关项目(项目编号20080311030-3)资助

摘　　要：选用萨福克、德国美利奴、特克塞尔、乌珠穆沁、以及右玉本地绵羊5个绵羊群体共250个个体,通过耳组织提取基因组DNA,用2对微卫星引物进行PCR扩增,通过电泳分型、凝胶成像系统分析各位点等位基因及全部个体的标记基因型,计算基因频率、多态信息含量(PIC)和杂合度等,从分子水平上分析5个绵羊品种的遗传多态性。结果表明,BM3413和OB2这2个位点的等位基因数分别为7和8,多态信息含量PIC﹥0.5,杂合度为0.8271～0.8467,均属于高度多态性位点,用于作为与生产性能相关的遗传标记是较理想的。In this study use a group of 250 sheep including Suffolk,Merino,Texel, Ujumuqin and Youyu local sheep, its population genetic polymorphism was analyzed in the molecular level by PCR and DNA extracted from their ears,fragment typing, allele of every locus and geno type analysis of all individuals by Gel Imaging system to calculate allele frequencies polymorphism information content （PIC） and Heterozygosity. The results showed that the number of alleles in BM3413 and OB2 was 7and 8, and the polymorphism information content PIC 〉 0.5, and the heterozygosity was 0.8 271-0.8 467. All of them were high polymorphicloci. So they were ideal genetic markers which were related to the production performance.

关 键 词：微卫星 杂合度 多态信息含量 有效等位基因 遗传距离 

分 类 号：S826[农业科学—畜牧学]