甜菜BvCK2基因全长cDNA的克隆和序列分析  

Molecular Cloning and Sequence Analysis of the Full-length cDNA of Gene BvCK2 from Beta vulgaris

作　　者：鲁振强[1] 潘彦遥[1] 陈连江[2] 朱延明[3] 李春宇[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080 [2]黑龙江大学农作物研究院,哈尔滨150080 [3]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国糖料》2009年第1期8-11,共4页Sugar Crops of China

基　　金：中国博士后科学基金面上资助项目(20080440918);黑龙江省博士后基金资助项目(LBH-Z07029);黑龙江省教育厅资助项目(11521222);黑龙江大学青年基金项目(QL200526)

摘　　要：甜菜M14品系在细胞胚胎学和遗传学特征上具有鲜明的无融合生殖现象。为了寻找在这一特殊的生殖过程中的相关基因及其调控作用,实验通过同源克隆及cDNA末端快速扩增(RACE)的方法,首次从甜菜M14品系中克隆了与生殖相关的基因BvCK2(Beta vulgaris casein kinase 2)的全长cDNA序列,长度为1 501 bp,开放阅读框为1002 bp,编码333个氨基酸。根据氨基酸序列计算蛋白分子量为39.28kDa,pI=8.16。同源比对表明,BvCK2与烟草CK2(GenBank No.AJ437635)的相似度为64.22%,与百合CK2(GenBank No.AF517838)的相似度为65.18%。通过细胞内定位分析,BvCK2所编码的蛋白主要存在于细胞核中。Beta vulgaris line M14 has an obvious characteristic of apomixis phenomenon in cell embryology and genetics. In order to find related genes in such process and its function, BvCK2 （Beta vulgaris casein kinase 2） gene was firstly cloned by using the method of homologous clone by the technique of Rapid Amplification of cDNA End （RACE）. The full-length cDNA sequence of BvCK2 is 1 501 bp in size and has an open reading frame of 1 002 bp encoding a polypeptide of 333 amino acids. The pI/protein molecular weight deduced from the amino acids sequence is 8.16 and 39.28 kDa. Homologous alignment indicates that it is 64.22% homology with tobacco （GenBank No. AJ437635）, 65.18% homology with lily （GenBank No. AF517838）. Prediction of protein localization sites （version6.4） indicates that the coding protein of BvCK2 gene mainly exists in nucleus.

关 键 词：甜菜 BvCK2 RACE 基因克隆 序列分析 

分 类 号：S566.303[农业科学—作物学]