p38丝裂原活化蛋白激酶在雄性小鼠生殖细胞株中的表达  

Expression of p38 mitogen-activated protein kinase in male mouse testicular cell lines

作　　者：马征兵[1] 杨瑛[1] 王珺[1] 罗亚宁[1] 闵保华[1] 张永奇[1] 

机构地区：[1]第四军医大学唐都医院妇产科,西安710038

出　　处：《重庆医学》2009年第3期295-296,共2页Chongqing medicine

摘　　要：目的检测p38丝裂原活化蛋白激酶(p38mitogen-activated protein kinase,p38MAPK)在雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中的表达,为深入研究p38MAPK基因在雄性小鼠精子发生中的作用机制奠定理论基础。方法应用逆转录-多聚合酶链反应(RT-PCR)检测雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中p38MAPK基因的表达。结果p38MAPK基因在TM4、GC-1spg、GC-2spd(ts)细胞株中均为阳性表达。结论p38MAPK基因的表达在雄性小鼠精子发生中可能发挥重要作用。Objective To detect the expression of p38 mitogen activated protein kinase（p38 MAPK） in male mouse testicular cell lines TM4, GC-1 spg and GC-2spd（ts）. It was the basis for us to further explore and study the possible relationship between p38 MAPK gene and male mouse spermatogenesis. Methods Reverse tanscriptase-polymerase chain reaction（RT-PCR） method was applied to detect the expression of p38 MAPK-mRNA in male mouse testicular cell lines TM4,GC-1spg and GC-2spd（ts）. Results p38 MAPK-mRNA was detected in male mouse testicular cell lines TM4,GC-1spg and GC-2spd（ts）. Conclusion The expression of p38 MAPK gene maybe play a very important role in male mouse spermatogenesis.

关 键 词：P38丝裂原活化蛋白激酶 精子发生 小鼠 

分 类 号：Q343[生物学—遗传学]