主栽品种镇稻88对水稻条纹叶枯的抗性特征及其遗传研究  

Mechanism and Inheritance of Resistance to Rice Stripe Disease in the japonica Rice Cultivar Zhendao 88

作　　者：周彤[1] 王磊[1] 程兆榜[1] 范永坚[1] 周益军[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国农业科学》2009年第1期103-109,共7页Scientia Agricultura Sinica

基　　金：江苏省农业科技自主创新资金项目(CX[08]129;CX[08]603);国家支撑计划(2006BAD02A16;2006BAD08A04);江苏省高技术项目(BG2006301);农业部行业专项(nyhyzx07-051);江苏省重大科技攻关项目(BE2005301);江苏省333工程人才项目资助

摘　　要：【目的】明确镇稻88对水稻条纹叶枯病的抗性特征及其抗性遗传模式,寻找与抗条纹叶枯病基因连锁的分子标记。【方法】利用单分蘖鉴定、苗期接种鉴定、非嗜性测验和抗生性测验分析镇稻88对水稻条纹叶枯病和介体灰飞虱的抗性;分别采用单分蘖鉴定和苗期接种鉴定方法对武育粳3号与镇稻88构建的F2群体、F2:3家系进行水稻条纹叶枯病抗性遗传分析;以SSR标记连锁分析方法对抗病基因进行定位。【结果】镇稻88表现出较强的病毒抗性和较弱的抗虫性;其对水稻条纹叶枯病的抗性表现为显性单基因的遗传模式;SSR标记分析显示该抗病基因位于水稻第11染色体上,与标记RM229间的遗传距离为4.7cM。【结论】镇稻88对水稻条纹叶枯病的抗性主要来自于对病毒的抗性,由一对主效核基因控制,以镇稻88为抗病亲本结合本研究结果,可望加快水稻条纹叶枯病抗性品种选育的速度。[Objective] The objective of this experiment was to analyze the resistance mechanism and resistance genetic model to rice stripe disease in Zhendao 88, and to screen the SSR marker related to the gene of rice stripe disease resistance in Zhendao 88. [Method] The resistance to rice stripe virus （RSV） and the vector, small brown planthopper （SBPH） （Laodelphax striatellus Fallen） was analyzed in Zhendao 88 by using the method of tiller inoculation, inoculation at the seeding stage, non-preference test and antibiosis test. The inheritance of rice stripe disease resistance in F2 lines and F2：3 lines from the cross Zhendao 88/Wuyujing No. 3 was studied by using tiller inoculation and inoculation at the seeding stage, respectively. The resistance gene was mapped based on SSR analysis. [Result] Zhendao 88 was highly resistant to rice stripe virus and weakly resistant to vector. Genetic analysis indicated that the resistance to rice stripe disease in Zhendao 88 was inherited as a single dominant trait. The rice stripe disease resistance gene in Zhendao 88 was located on the chromosome 11, with the distance of 4.7cM from a SSR marker RM229. [Conclusion] The rice stripe disease resistance in Zhendao 88 was mainly derived from the resistance to virus and controlled by a single major gene. The breeding process for rice stripe disease resistance should be accelerated by using Zhendao 88 as a resistance parent.

关 键 词：水稻条纹叶枯病 水稻品种 抗性特征 遗传分析 基因定位 

分 类 号：S511.22[农业科学—作物学]