轮套作对黄瓜土壤微生物多样性及产量的影响  

Effect of Rotation and Interplanting on Cucumber Yield and Soil Microbial Diversity

作　　者：庄岩[1] 吴凤芝[1] 杨阳[1] 尚庆茂[2] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国农业科学》2009年第1期204-209,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30571264);国家“863”计划项目(2006AA10Z421)

摘　　要：【目的】研究轮套作对黄瓜各个时期的土壤微生物多样性及黄瓜产量的影响。【方法】采用随机扩增多态性DNA分析(random amplified polymorphism DNA,RAPD)方法。【结果】轮作处理中,小麦和大豆轮作处理可以显著提高黄瓜土壤微生物群落DNA序列多样性各指数(P<0.05),其黄瓜产量极显著高于对照(P<0.01);与对照相比,毛葱套作处理显著提高黄瓜土壤微生物群落DNA序列多样性各指数,其黄瓜产量极显著高于对照(P<0.01)。【结论】小麦、大豆轮作和毛葱套作有利于改善土壤微生态环境,提高黄瓜产量。[Objective] The objective of the experiment is to study the effect of rotation and interplanting on microbial community diversity in different growth periods and cucumber yield. [Method] RAPD techniques were adopted as research methods. [Result] Wheat-soybean rotation significantly improved the cucumber microbial DNA sequence diversity index （P〈 0.05）, and significantly increased yield of cucumber （P〈0.01）.Compared to control, onion interplanting treatment also significantly increased cucumber microbial DNA sequence diversity index and yield of cucumber （P〈0.01）. [Conclusion] Wheat-soybean rotation and garlic interplanting could facilitate improving soil micro-ecological environment and increase the yield of cucumber.

关 键 词：黄瓜 轮套作 土壤微生物群落 DNA序列多样性 产量 RAPD 

分 类 号：S642.2[农业科学—蔬菜学] S154.3[农业科学—园艺学]