猪HB-EGF基因多态性与产仔数的关联性分析  

Analysis of the Association Between Polymorphisms of HB-EGF Gene and Litter Size in Pigs

作　　者：马喜山[1] 王爱国[1] 刘桂芬[1] 傅金恋[1] 

机构地区：[1]中国农业大学动物科学技术学院/农业部畜禽遗传育种重点开放实验室,北京100193

出　　处：《中国农业科学》2009年第1期274-282,共9页Scientia Agricultura Sinica

基　　金：国家高技术研究发展计划项目(2007AA10Z166);国家自然科学基金(30771540)

摘　　要：【目的】研究猪HB-EGF基因的多态性及其与产仔数的关系,寻找与猪产仔数相关的分子遗传标记,以加快猪育种进程。【方法】利用PCR-SSCP技术研究HB-EGF基因外显子多态性,利用最小二乘均数法对其与产仔数的关系进行分析,同时对两个多态位点的方差组分进行了分析。【结果】在两对引物(E5和E7)中各检测到一个多态位点,均出现3种基因型(AA、BB、AB)。E5位点:对于大白猪,在3～8胎中,BB型个体的TNB和NBA均显著高于AA型个体(P<0.05),在所有胎次中,BB型个体的TNB显著高于AA型个体(P<0.05),NBA极显著高于AA型个体(P<0.01),TNB和NBA的遗传主要受基因加性效应的影响;对于长白猪,在4个阶段中3种基因型个体的TNB和NBA之间差异不显著(P>0.05)。E7位点:对于大白猪,在3～8胎和所有胎次中,AA型个体的TNB均显著高于BB型个体(P<0.05),TNB的遗传主要受基因加性效应的影响;对于长白猪,在3～8胎和所有胎次中,AB型个体的NBA均显著高于BB型个体。【结论】对于大白猪,E5位点BB基因型可作为在TNB和NBA性状上的标记基因型,E7位点BB基因型可以作为TNB性状的标记基因型。[Objective] The pig HB-EGF gene was used to study the polymorphism, and the association between the polymorphism and litter size was analyzed in order to find the molecular marker being used in marker-assisted selection（MAS） to accelerate the swine breeding progress. [Method] The polymorphism of HB-EGF gene was studied by PCR-SSCP, which the association between and litter size was studied by the least square mean, and the variance components at the two polymorphisms were also analyzed. [Result] There were two polymorphisms occurring in exon5 （primer E5） and exon6 （primer E7） respectively, each one with three genotypes （AA, BB and AB）. At E5, in Large White, BB genotype had significantly higher （P〈0.05） TNB and NBA than AA genotype in third to eighth parity, and in all parities, BB genotype had significantly higher （P〈0.05） NBA than AA genotype, while had greatly significantly higher TNB than AA （P〈0.01）, the heredity of TNB and NBA was mostly influenced by the genetic additive effect; In Landrace, there was no significant difference on TNB and NBA in the four phases. At E7, in Large White, AA genotype had significantly higher （P〈0.05） TNB than BB genotype in third to eighth parity and all parities, the heredity of TNB was mostly influenced by the genetic additive effect; in Landrace, AB genotype had significantly higher （P〈0.05） NBA than BB genotype in third to eighth parity and all parities. [Conclusion] In Large White, the BB genotype at E5 was favorable marker genotype for TNB and NBA, and the BB genotype at E7 was favorable marker genotype for TNB.

关 键 词：猪 肝素结合性表皮生长因子 多态性 产仔数 

分 类 号：S828[农业科学—畜牧学]