细胞松弛素B和鬼笔环肽对梨花粉萌发和花粉管生长的影响  

Effects of Cytochalasin B and Phalloidin on Pyrus pyrifolia Self-incompatible Pollen Germination and Tube Growth

作　　者：刘珠琴[1,2] 姜雪婷[1] 徐国华[3] 张绍铃[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]宁波市农业科学研究院林业所,浙江宁波315040 [3]江苏教育学院生物系,南京210013

出　　处：《西北植物学报》2008年第12期2393-2399,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：高校博士学科点专项科研基金项目(B200523)资助

摘　　要：以砂梨(Pyrus pyrifoliaNakai)品种今村秋(Imamuraaki)和丰水(Hosui)为材料,分别用光学显微镜和荧光显微镜观察了离体和半活体条件下微丝骨架解聚剂细胞松弛素B(cytochalasin B,CB)和稳定剂鬼笔环肽(phalloidin)对梨花粉萌发和花粉管生长的影响.结果表明:(1)低浓度(10μg/mL)鬼笔环肽能促进花粉萌发和花粉管生长,但高浓度对花粉萌发和花粉管的生长具有抑制作用;CB抑制花粉萌发和花粉管生长,且抑制效应随其浓度的增加而增强.(2)鬼笔环肽处理柱头后进行自花授粉,可明显促进自花花粉萌发和花粉管的生长,而CB处理柱头后异花授粉则抑制异花花粉萌发及其花粉管生长.可见,微丝骨架参与了梨花粉萌发和花粉管生长过程,并参与了梨自交不亲和反应的调节.The effects of actin cytoskeleton depolymerized reagent cytochalasin B and stabile reagent phalloidin on pollen germination and tube growth have been studied in vivo and in vitro by optical microscopy and fluorescence microscopy,respectively.The results showed：（1）lower concentration of phalloidin（10 μg/mL） could promote pollen germination and tube growth,but high concentration inhibited germination and growth,CB inhibited pollen germination and tube growth,and the higher was the concentration,the stronger was the inhibitory effect.（2）Lower concentration of phalloidin also promoted self-pollen germination and tube growth after self-pollination in vitro,while CB inhibited pollen germination and tube growth after cross-pollination.The results showed that actin cytoskeleton was involved in Pyrus pyrifolia pollen germination and tube growth,and might play a role in Pyrus pyrifolia self-incompatibility response.

关 键 词：梨 微丝骨架 鬼笔环肽 细胞松弛素B 花粉 花粉管 

分 类 号：Q945.6[生物学—植物学]