抗子宫内膜抗体对人早孕绒毛滋养细胞凋亡及FasL蛋白表达的影响  

Effects of anti-endometrium antibody on AI and FasL expression of cultured human cytotrophoblast cells

作　　者：王永萍[1] 徐永萍[1] 邱健青[1] 马小静[1] 

机构地区：[1]山东大学第二医院妇产科,济南250033

出　　处：《现代妇产科进展》2008年第12期925-928,共4页Progress in Obstetrics and Gynecology

基　　金：山东省计生委科研基金资助项目

摘　　要：目的:探讨体外培养人早孕绒毛滋养层细胞在游离抗子宫内膜抗体(Em-Ab)环境中,细胞凋亡指数(apoptosis index,AI)及凋亡相关因子配体(factor associated suicide ligend,FasL)的表达。方法:培养经胰蛋白酶/DNA酶Ⅰ联合消化,通过Percoll细胞分离液纯化得到绒毛滋养细胞。用TUNEL法测定EmAb(+)血清处理组和EmAb(-)血清处理组绒毛滋养细胞培养0、48、72、96h时的细胞凋亡指数,同时用免疫细胞化学法测定FasL蛋白的表达。结果:EmAb(+)血清处理组于培养72、96h时间点测得的AI明显高于EmAb(-)血清处理组(P<0.01);FasL蛋白表达明显低于EmAb(-)血清处理组;而培养48h AI及FasL蛋白表达无显著差异。结论:绒毛滋养细胞在EmAb(+)环境下AI增加,FasL蛋白表达减少。Objective：To investigate apoplosis index（AI） and factor associated suicide ligend（FasL） expression of cultured human cytotrophoblast cells under free EmAb environment. Methods： Chorionic villi tissue of normal 6 - 8 week pregnancy were obtained. Cytotrophoblast ceils were collected by trypsin-DNase I digestion and Percoll gradient centrifugation. They were cultured under EmAb（ ＋ ） serum group and EmAb（-） serum group for 0,48,72,96h. AI and FasL expression was observed by TUNEL and immunohistochemistry respectively. Results： AI in EmAb（ ＋ ） serum group was significantly higher than that of EmAb（-） serum group（P 〈 0.05 ）. FasL concentrations in EmAb （ ＋ ） serum group were significantly lower than those of EmAb（-） serum group when measured at 72h,96h culture period. AI and FasL concentrations were not obviously different when measured at 48h culture period （ P 〉 0.05 ）. Conclusion： AI of cytotrophoblast cells increased and FasL expression decreased when they were cultured under free EmAb environment.

关 键 词：绒毛滋养细胞 细胞凋亡指数 凋亡相关因子配体 

分 类 号：R714.21[医药卫生—妇产科学]