The effect of maternal BDE-209 exposure on offsping's expression of MAP-1B and S100 in hippocampus of the rats  

作　　者：Yu Lin Su Tao Chen Shengqiang Chen Dunjin 

机构地区：[1]The Research Institute of Gynecology and Obstetrics,the Third Hospital of Guangzhou Medical College,Guangzhou 510150 [2]The Research Institute of Neuroscience,the Second Affiliated Hospital of Guangzhou Medical College

出　　处：《现代妇产科进展》2008年第12期957-960,共4页Progress in Obstetrics and Gynecology

摘　　要：Objective:To study the effect of maternal BDE-209 (brominated Diphenyl Ethers-209)exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat's hippocampus of the offspring by RT-PCR.Methods:Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg/(kg·d) by oral gavage throughout gestation and lactation in experimental group.The control group was administered only with the same capacity of peanut oil at the same time.The expression of MAP-1B in the hippocampus of the offspring's rats were tested when the pups were newborn,7days,14 days,21days and 45days old respectively by means of RT-PCR.Result:MAP-1B protein showed a statistically significantly lower concentration in the groups 14 days,21days,45days than that of the control groups.The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups.But only the 45days groups had significant difference of expression of MAP-1B protein compared with the control groups(P<0.05).Conclusions:Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1b protein in hippocampus of offspring's rats.Objective：To study the effect of maternal BDE-209 （brominated Diphenyl Ethers-209） exposure on the expression of microtubule-associated protein-lb （map-1b） and S- 100 in rat＇s hippocampus of the offspring by RT-PCR. Methods：Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg/（ kg · d） by oral gavage throughout gestation and lactation in experimental group. The control group was administered only with the same capacity of peanut oil at the same time. The expression of MAP-1B in the hippoeampus of the offspring＇s rats were tested when the pups were newborn,7days, 14 days , 21days and 45days old respectively by means of RT-PCR. Result： MAP-1B protein showed a statistically significantly lower concentration in the groups 14 days,21 days ,45days than that of the control groups. The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups. But only the 45days groups had significant difference of expression of MAP-1B protein compared with the control groups （ P 〈 0.05 ）. Conclusions： Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1 b protein in hippocampus of offspring＇s rats.

关 键 词：产妇 溴化二苯醚 哺乳期 药物分析 

分 类 号：R473.71[医药卫生—护理学] R995[医药卫生—临床医学]