马内菲青霉蛋白酶的体外诱导及其活性测定  

In vitro induction of proteinase production of Penicillium marneffei and measurement of its activity

作　　者：李民[1] 杨连娟[1] 章伟[1] 章强强[2] 

机构地区：[1]上海市皮肤病性病医院,上海200050 [2]复旦大学附属华山医院皮肤科真菌室,上海200040

出　　处：《微生物与感染》2008年第4期224-225,230,共3页Journal of Microbes and Infections

摘　　要：目的建立马内菲青霉蛋白酶的体外诱导方法并进行活性测定。方法制作酶诱导培养基,在不同pH条件下培养马内菲青霉,在280nm紫外光下分光光度法检测不同诱导时间的蛋白酶活性。结果成功诱导并定量测定了马内菲青霉在不同pH条件下、不同诱导时间的体外蛋白酶产出量。pH4.0时,菌株在第6天出现分泌高峰;pH5.6时,菌株在第3天出现分泌高峰;而pH7.2时,菌株几乎无蛋白酶产出。结论建立了马内菲青霉蛋白酶产出的体外诱导和检测方法,为进一步研究马内菲青霉体外蛋白酶的理化特性提供了途径。Objective To establish a method of inducing proteinase production of Penicillium marneffei in vitro and to measure its proteinase activity. Methods The B-6323 strain of P. marneffei was cultured in proteinase inducing medium under different pH conditions, and the production of proteinase over the course of several days under different pH conditions was measured using a spectrophotometric method （280 nm UV light）. Results Proteinase was induced and it＇ s activity was measured over the course of several days under different pH conditions. The strain had the highest proteinase activity on day 6 at pH 4. 0; the highest proteinase activity on day 3 at pH 5.6 ; and no proteinase activity at pH 7. 2 on any day. Conclusion A method of inducing proteinase production of P. marneffei in vitro and measuring its activity was established, and may be a method to be used for future research to understand the biochemical properties of proteinase.

关 键 词：马内菲青霉菌 蛋白酶 分光光度法 

分 类 号：R379[医药卫生—病原生物学]