小麦种子纯度的分子标记检测方法  

Method of Wheat Seeds Purity Testing by Molecular Markers

作　　者：王立新[1] 常利芳[1] 李宏博[1] 葛玲玲[1] 信爱华[1] 高世庆[1] 季伟[1] 孙辉[1] 赵昌平[1] 

机构地区：[1]北京市农林科学院,北京杂交小麦工程技术研究中心,北京100097

出　　处：《麦类作物学报》2009年第1期1-8,共8页Journal of Triticeae Crops

基　　金：北京市农业育种基础研究创新平台项目(YZPT01-06)

摘　　要：为了探索一种准确评价小麦品种种子纯度的技术体系,在比对了400个小麦品种的SSR、EST-SSR和AFLP-SCAR指纹以及对上千份种子进行纯度检测的基础上,提出了检测种子纯度的策略:(1)联合使用SSR、EST-SSR和AFLP-SCAR标记检测种子纯度,并推荐了7对SSR引物、5对EST-SSR引物和3对AFLP-SCAR引物为检测种子纯度的核心引物;(2)在待测品种的种子之间比对核心分子标记位点的带型,当某(些)个体与多数个体之间≥3位点的带型不同时,方可判定为杂株。此外,本文还就如何提高种子纯度检测的工作效率、降低检测成本,提出了合理利用核心引物和合理混合DNA样品的方法。Seed purity is an important criterion of wheat （Triticum aestivum L. ） seed quality. A precise testing method can estimate seed quality accurately. After comparing DNA fingerprints of 400 cultivars and testing seed purity for more then 1 000 wheat cultivars, we recommended a method to test wheat seed purity using molecular markers, including seven pairs of SSR primers, five pairs of EST-SSR primers and three pairs of AFLP-SCAR primers as core primers. According to differences of DNA pattern of core marker loci and genetic similarity between 400 cultivars, three or more than three differences of DNA pattern of core marker loci among individuals of tested sample could be an index to differentiate wrong seed from tested cultivar. To save money and time in the test, here, reasonable primers using and DNA sample mixture were recommended. The result will be benefit for the test of wheat seed purity.

关 键 词：普通小麦 种子纯度 分子标记 

分 类 号：S512.1[农业科学—作物学] S330