反相高效液相色谱法测定5-氨基水杨酸及其代谢物在人血浆及尿中的浓度  

RP HPLC determination of 5 aminosalicylic acid(5 ASA) and its metabolite in plasma and urine of human

作　　者：陆志城[1] 蔡杏春[2] 刘文坚[2] 

机构地区：[1]广州医学院第二附属医院 [2]广州市医药工业研究所药物代谢研究室

出　　处：《中国医院药学杂志》1998年第6期248-250,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：研究５－氨基水杨酸及其代谢物在人血浆及尿中的浓度。方法：反相高效液相色谱法（ＲＰ－ＨＰＬＣ）为测定方法。结果：血浆测定两者线性范围均为０．０８～８．００μｇ·ｍｌ－１，最低检出浓度均为０．０４μｇ·ｍｌ－１，两者平均回收率分别为８７．９３％，９１．４８％，日内ＲＳＤ分别为６．０４％，５．５１％，日间ＲＳＤ分别为７．９８％，４．４３％。５－氨基水杨酸尿浓度测定线性范围为０．５～１０μｇ·ｍｌ－１，最低检出浓度为０．２５μｇ·ｍｌ－１，平均回收率为１０１．８３％，日内ＲＳＤ为１．５７％，日间ＲＳＤ为２．６４％。结论：该测定方法快速，简便。n RP HPLC method for the determination of 5 aminosalicylic acid(5 ASA) and its metabolite, acetyl 5 ASA(AC 5 ASA), in plasma and urine of human was described. The standard curves of 5 ASA and AC 5 ASA were linear within the range of 0.08 ￣ 8.00 μg·ml -1 in plasma, 0.5 ￣10 μg·ml -1 (5 ASA) in urine. Their detection limits in plasma were 0.04 μg·ml -1 and 0.25 μg·ml -1 (5 ASA) in urine. The recoveries of 5 ASA and AC 5 ASA in plasma were 87.93% 91.48% , respectively, and in urine was 101.83% . The RSD of within day and between day of 5 ASA and AC 5 ASA in plasma were 6.04% , 5.51% ; 7.98% , 4.43% , respectively. 5 ASA in urine was 1.57% and 2.64% .

关 键 词：反相 高效液相色谱法 5-氨基水杨酸 代谢物 

分 类 号：R971.1[医药卫生—药品] R969.1[医药卫生—药学]