钙拮抗剂尼群地平的反相高效液相色谱分析及其在家兔体内的分布  

RP-HPLC ANALYSIS OF NITRENDIPINE AND ITS DISTRIBUTION STUDY IN RABBITS

作　　者：傅强[1] 贺浪冲[1] 扬广德 常春[1] 武玥 

机构地区：[1]西安药学院药物分析教研室,西安710061

出　　处：《西安医科大学学报》1998年第2期276-279,共4页Journal of Xi'an Medical University(Chinese)

基　　金：陕西省自然科学基金

摘　　要：对兔血浆及组织器官中钙拮抗剂尼群地平的分析方法进行了研究，建立了反相高效液相色谱（RP-HPLC）的内标测定法。样品在弱碱性条件下经环已烷-异丙醇（96．5：3．5）提取，用YWGC18反相柱，以地尔硫作内标，甲醇：50mmol／L磷酸盐缓冲液（70：30）pH6．8为流动相，UV236nm检测。方法最低检测限约为10ng，血药浓度在21．6～216μg／L之间，尼群地平的量与其峰面积比值之间，线性关系良好，回归方程为Y＝0．00672X－0．013，r＝0．9958。方法回收率95．4％～98．1％，RSD＜4．63％。　用该分析方法测定了家兔口服尼群地平2h后在主要脏器体液中的分布。A analytical method for determination of nitrendipine, a calcium antagonist, in serum,tissues and organs of rabbits was developed. Inweak alkaline conditions, samples were extractedwith cyclohexane-isopropanol (96. 5: 3. 5. v/v ) and analyzed with YWG C18 reversed phase column at uv236um. Diltiazem was used as internal standard and methanol: 50mmol/L phosphate buffer(70: 30v/v)was used as mobile phase. The minimum detectionlimit of nitrendipine was 10ng. The liner range wasbetween 21. 6 ～ 216ng/ml with regression equationY = 0.00673X - 0.013. r = 0. 9958. The analyticalrecoveries were 95. 4% ～98. 1% with RSD less than4. 63 %. This analytical procedure was employed todetermin nitrendipine distribution in rabbits 2 hoursafter the drug was administered.

关 键 词：尼群地平 钙拮抗剂 高效液相色谱法 体内分布 

分 类 号：R972.4[医药卫生—药品]