广西地区壮族2型糖尿病患者脂联素基因多态性研究  

ASSOCIATION OF THE T-G POLYMORPHISM IN ADIPONECTIN(EXON 2) WITH DIABETES IN ZHUANG PEOPLE IN GUANGXI

作　　者：陈青云[1] 苏庆建[2] 覃伟武[3] 戴霞[1] 罗佐杰[1] 蒙碧辉[1] 吴耀生[4] 黄松[1] 冼晶[1] 赖璐华[1] 覃家锦[1] 

机构地区：[1]广西医科大学第一附属医院内分泌科,南宁530021 [2]广西南宁市第二人民医院内分泌科 [3]广西医科大学第一附属医院核医学科 [4]广西医科大学生化教研室

出　　处：《广西医科大学学报》2008年第6期821-824,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金项目(No.30260042);广西自然科学基金项目(No.桂科基0575070)

摘　　要：目的：研究脂联素基因外显子2单核苷酸多态性（SNP）＋45T→G多态性与广西地区壮族2型糖尿病（T2DM）、肥胖及胰岛素抵抗（IR）的关系。方法：运用PCR-RFLP的方法对303例无亲缘关系的广西地区壮族人群[正常糖耐量者（NGT）98例、糖耐量减低者（IGT）108例、T2DM者97例]中检测脂联素基因外显子2的多态性。结果：广西地区壮族人群中脂联素基因＋45位有SNP＋45T→G存在。含脂联素基因外显子2的SNP＋45GG基因型的人群BMI、FPG、PPG、FINS、HOMA-IR高于含TT基因型的人群（P〈0.05）.T2DM组的含GG基因型人数高于NGT、IGT组，GG基因型比TT基因型增加了3.077倍T2DM的危险性（95％CI：1.240-7.634）。结论：脂联素基因外显子2SNP＋45GG变异与肥胖、IR及T2DM相关。Objective： To evaluate the relationship between single nucleotide polymorphism （SNP）＋45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus （T2DM）, obesity or insulin resistance （IR） in Zhuang people in Guangxi. Methods： Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed to identify the T-G polymorphism in adiponectin （exon 2） in 303 unrelated Zhuang people in Guangxi, including 98 normal glucose tolerance, 108 impaired glucose tolerance and 97 type 2 diabetes mellitus. Result： In Zhuang people of Guangxi, ＋45T→G polymorphism in adiponectin gene were found. The SNP＋45 GG genotypes in adiponectin gene had significantly higher BMI, FPG, PPG, FINS and HOMA-IR levels than TT genotypes in Zhuang people（P〈0.05）, and GG genotype had significantly higher T2DM than that in NGT and IGT groups. Subjects with the GG genotype at position 45 had a significantly increased risk of T2DM （odds ratio 3.077[95%CI： 1.240-7.634]） ,compared with those having the TT genotype at position 45. Conclusion： The SNP ＋45 of adiponectin gene is associated with o- besity, IR and T2DM.

关 键 词：脂联素 基因多态性 外显子2 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]