ω-3多不饱和脂肪酸治疗去卵巢大鼠骨质疏松对血脂的影响  

Effect of ω-3 polyunsaturated fatty acids on the serum lipid of ovariectomized rats

作　　者：马戎[1] 许萍[2] 常小霞[3] 吴洁[3] 翟青新[4] 沈小志[2] 刘宝瑞[5] 

机构地区：[1]南京大学医学院附属鼓楼医院普通外科,江苏南京210008 [2]南京大学医学院附属鼓楼医院营养科,江苏南京210008 [3]江苏省人民医院妇女保健科,江苏南京210036 [4]南京大学医学院附属鼓楼医院动物实验中心,江苏南京210008 [5]南京大学医学院附属鼓楼医院肿瘤科,江苏南京210008

出　　处：《肠外与肠内营养》2009年第1期4-6,共3页Parenteral & Enteral Nutrition

基　　金：南京市卫生局科研课题资助(YKK05124;YKK05101)

摘　　要：目的:观察ω-3多不饱和脂肪酸(ω-3 PUFA)治疗去卵巢大鼠骨质疏松对血脂的影响。方法:60只3月龄未交配的雌性SD大鼠,腹腔手术切除双侧卵巢或等体积脂肪,分为去卵巢组(OVX组)、17α-炔雌醇组(E2组)、ω-3 PUFA小剂量组(A组)、ω-3 PUFA中剂量组(B组)、ω-3 PUFA大剂量组(C组),假手术组(Sham组)为对照。用药90 d。采血测定血清TC、TG、HDL-C、LDL-C,并对各组血脂测定值进行比较。结果:OVX组TC、LDL-C明显高于Sham组(P<0.05,P<0.01),TG、HDL-C、HDL-C/TC无显著性差异;E2组TC高于Sham组(P<0.05),HDL-C、LDL-C、HDL-C/TC均低于OVX组(P<0.05);实验药物组(A、B、C组)大鼠HDL-C/TC显著高于E2组(P<0.01)。结论:对去卵巢大鼠用ω-3 PUFA治疗骨质疏松,对血脂的影响明显优于炔雌醇组。Objective ovariectomized rats ： To study the effect of ω-3 polyunsaturated fatty（ to-3 PUFA） on the serum lipid of Methods ： 60 virgin female Sprague-Dawley rats, 3 months of age, were randomized by the stratified weighl method into 6 groups： Sham group ：deionized water 2 mL/d, OVX group： eionized water 2 mL/d, E2 group： OVX ＋ 17α-ethinylestradiol O. 1 mg/（kg· d） , A group： OVX ＋ ω-3 PUFA 75 mg/（ kg·d）, B group ： OVX ＋ ω-3 PUFA 150 mg/（ kg · d） and C group ： OVX ＋ ω-3 PUFA 300 rag/ （kg·d）. Each group had 10 rats. Experiment period was 90 days. Body weight were measured every week. Serum were collected to measure Cholesterol （ TC ）, Triglyceride （ TG）, High-density lipoprotein- cholesterol （ HDL-C）, and Low-density lipoprotein-cholesterol （LDL-C）. Results ： There were significant difference between groups in the serum lipid. In the OVX group, TC and LDL-C increased. In the E2 group, TC increased compared with that in the Sham group, and HDL-C, LDL-C and HDL-C/TC decreased significantly compared with that in the OVX group. In the A, B and C groups HDL-C/TC increased significantly compared with those in the E2 group. Conclusion： The ovariectomized rats have lip id metabolism disorderd. The ω-3 PUFA and estrogen replacement therapy plays a role in the lipid metabolism. The ω-3 PUFA has the best efficiency in reducing the level of serum lipid .

关 键 词：Ω-3多不饱和脂肪酸 去卵巢大鼠 血脂 

分 类 号：R580[医药卫生—内分泌] R96[医药卫生—内科学]