移植后淋巴组织增生性疾病动物模型的建立和鉴定  

Establishment and identification of post-transplantation lymphoproliferative disorders(PTLD) model

作　　者：董启超[1] 许世峰[1] 周旭[1] 刘军[1] 

机构地区：[1]山东大学附属省立医院器官移植中心肝胆外二科,山东济南250021

出　　处：《中国普通外科杂志》2009年第1期36-39,共4页China Journal of General Surgery

基　　金：山东省优秀中青年奖励基金资助项目(2005BS03006)

摘　　要：目的探索建立移植后淋巴组织增生性疾病（PTLD）动物模型的方法。方法将40名EB病毒（EBV）阳性志愿者的外周血淋巴细胞（huPBL）分别经腹腔注射入3只联合免疫缺陷动物即SCID小鼠体内,通过血液人免疫球蛋白（IgG）的测定,确定人类免疫细胞在小鼠体内的重建。在小鼠濒死或死亡后取出肿瘤组织,通过组织病理切片和免疫组化的分析方法确定淋巴增生性疾病（LPD）的产生及其细胞来源。结果移植huPBL后8周和12周SCID小鼠血清中人IgG含量分别达到（750.0±13.2）μg/mL和（1 050.0±14.6）μg/mL。濒死或死后,组织病理切片显示为伴有高度有丝分裂率的大细胞淋巴瘤,肿瘤CD20阳性,LMP-1阳性。在40名志愿者中,10例在8周后产生LPD（25%）,SCID小鼠的中位成瘤率为100%;8例在10～16周出现LPD（20%）,中位成瘤率为55%;其他22例（55%）在16周后仍无LPD产生。结论SCID小鼠腹腔注射EB病毒阳性的人类外周血白细胞可建立PTLD的动物模型。Objective To explore the feasibility of constructing PTLD model. Methods The peripheral blood lymphocytes of 40 EBV （ ＋ ） volunteers were injected intraperitoneally into 3 SCID mice respectively. IgG determination was utilized to confirm the reestablishment of human immunocell in SCID mice. The tumor tissue of the dying or dead mice were retrieved, and then the establishment of LPD model was determined by pathological section and immunohistochemistry. Results The IgG level in the blood serum of SCID mice was （750.0 ±13. 2） μg/mL and （ 1 050. 0 ± 14. 6） μg/mL respectively at 8 weeks and 12 weeks post transplantation. Pathological section of retrieved tumor tissue indicated that the tissue was large-cell lymphoma with high mitotic rates. The tumors were CD20 positive and LMP-1 positive. Among the 40 volunteers, human peripheral blood lymphocyte from 10 volunteers （25%） produced LPD rapidly（ median time to LPD, 8 weeks）and with high penetrance （median, 100% ） ; whereas 20% （8 of 40 volunteers） developed LPD tumor later （ median 12 weeks ） , and only in partial mice （ median penetrance, 55 % ） ; HuPBLs from the remainder of the donors （22 of 40,55 % ） produced no LPD after 16 weeks. Conclusions PTLD model can be successfully established by intraperitoneal injection of EBV （ ＋ ）huPBL in SCID mice.

关 键 词：淋巴组织增生性疾病 模型 动物 小鼠 SCID EB病毒 

分 类 号：R392[医药卫生—免疫学]