改良四氯化碳皮下注射法制备犬肝硬化门静脉高压症模型  

A modified liver cirrhosis portal hypertension model in canines induced by subcutaneous injection of carbon tetrachloride

作　　者：陈钟[1] 戴向华[1] 明志祥[1] 朱李瑢[1] 

机构地区：[1]南通大学附属医院肝胆外科,江苏南通226001

出　　处：《中国普通外科杂志》2009年第1期63-67,共5页China Journal of General Surgery

基　　金：江苏省南通市社会发展基金(S5044)

摘　　要：目的寻找制作一种理想的适合于外科研究的大型动物肝硬化门静脉高压症模型。方法犬20只,用60%CCl4菜籽油乳剂于犬背部皮下注射,1.0～1.3 mL/kg,每10天注射1次,共6～8次。配合饮食控制,所有犬以10%的猪油米饭喂养,注射CCl4菜籽油乳剂后4 d内控制米饭量每日15 g/kg,第5～10天不控制饮食量。造模中观察犬的一般状态,检测肝功能指标血清谷丙转氨酶（ALT）,血清总蛋白（TP）,清蛋白（ALB）,球蛋白（G）,A/G的变化;彩色多普勒监测肝脏形态、门静脉直径及血流变化。每2周手术切取小块肝组织活检,光镜下观察肝脏病理变化。结果造模中犬体重持续性下降。ALT早中期上升,后期有所下降,TP及ALB持续性下降,G变化不明显,A/G持续性下降。彩色多普勒检查示,注药8周后肝脏进行性缩小,质地不均匀,颗粒逐渐变粗,肝包膜不平滑;门静脉直径逐渐变粗,门静脉血流逐渐变缓。光镜检查10～12周犬肝脏假小叶形成。犬死亡3只,死亡率15.0%。结论皮下注射四氯化碳10～12周形成的犬肝硬化门静脉高压症模型,方法简便,成功率高,适用于外科研究。Objective To find an ideal liver cirrhosis portal hypertension model in big animals suitable for surgical study. Methods Twenty canines were treated by subcutaneous injection of 60% CCl4 colza oil emulsion into the back, at a dose of 1.0- 1.3 mL/kg every 10 days for a total of 6 -8 times. At the same time, it was combined with the control of food intake. All canines were fed with rice mixed with 10 % hog fat. The amount of rice was 15 g/kg per day from the first day to the forth day, but the amount of food was not controlled from the fifth day to the tenth day. During the process, the canines＇ general condition was observed and the changes of hepatic functions such as ALT, TP, ALB, G and A/G were measured. The changes of liver morphology, the diameter and blood flow of portal vein were monitored with color Doppler. Every two weeks, a piece of hepatic tissue to observe the pathological change of liver was excised operatively. Results During the process, the body weight of the canines decreased continually; ALT increased during early and middle stages and decreased during advanced stage; TP and ALB always decreased, and A/G continuously decreased, but G did not change significamtly. Eight weeks after injection, liver became progressively smaller, its density was not well-distributed, and liver particles gradually became coarse, and liver capsule became rough. The diameter of portal veins became larger and the velocity of portal vein became slower. Liver cirrhosis with psudolobules was found on light microscopy from the tenth week to the twelfth week. Three canines died, with mortality of 15.0 % . Conclusions It took 10 - 12 weeks to establish this liver cirrhosis portal hypertension models in canines by subcutaneous injection of CCl4. This method is convenient, has high success rate, and is suitable for use in surgical research.

关 键 词：肝硬化 高血压 门静脉 四氯化碳 模型 动物 犬 

分 类 号：R657.3[医药卫生—外科学]