双歧杆菌与乳酸杆菌在肾衰大鼠胃肠道不同部位的定植及其对小分子毒素的分解  

Field planting of Bacillus bifidus and Bacillus acidi lactic on various gastrointestinal sites and their decomposition of low molecule urotoxins in rats with renal failure

作　　者：熊文[1] 蒋哲峰[2] 蒋云生[1] 贺丽娟[1] 

机构地区：[1]中南大学湘雅二医院肾内科,长沙410011 [2]中南大学湘雅二医院检验科,长沙410011

出　　处：《中南大学学报（医学版）》2009年第1期35-39,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30670974)~~

摘　　要：目的:观察双歧杆菌与乳酸杆菌在慢性肾衰大鼠胃、空肠、回肠、升结肠和降结肠黏膜的定植情况,及其对肌酐(Cr)、尿素氮(UN)、尿酸(UA)的分解作用。方法:SD大鼠40只分为4组,A组为正常对照组10只行肾脏假手术,30只大鼠作5/6肾切除后分成B组为病理对照组,C组和D组为治疗组,分别喂饲双歧杆菌和乳酸杆菌。1周后处死动物留取胃肠黏膜作细菌计数,并测定各段消化液及血中尿素氮、肌酐、尿酸浓度。结果:B组大鼠胃、空肠、回肠、升结肠和降结肠黏膜双歧杆菌与乳酸杆菌定植数低于A组,差异有统计学意义(P<0.05);C,D两组黏膜双歧杆菌与乳酸杆菌定植数高于B组和A组(P<0.05),且胃肠道各段黏膜双歧杆菌和乳酸杆菌计数之间差异有统计学意义(P<0.05),胃黏膜细菌数最少,降结肠黏膜细菌数最多;C,D两组各段消化液尿素氮、肌酐、尿酸水平低于B组(P<0.05),各段之间差异有统计学意义(P<0.05);C,D两组血清尿素氮、肌酐、尿酸水平低于B组(P<0.05)。消化道各段乳酸杆菌及双歧杆菌的定植数与血液及消化液中尿素氮、肌酐和尿酸均呈明显负相关。结论:消化道不同部位双歧杆菌和乳酸杆菌定植及各段消化液小分子尿毒素浓度存在差异;喂饲这两种细菌能降低肾衰大鼠血中小分子尿毒素浓度。Objective To investigate the field planting of Bacillus bifidus and Bacillus acid lactic on mucosa membrane of gaster, jejunal, ileum, ascending colon, and decending colon in rats with renal failure, and to observe their decompositon of creatinine （Cr）, urea nitrogen （UN）, and uric acid （ UA ）. Methods Forty SD rats were randomly divided into 4 groups. Group A was normal control group, and 10 of them were sham-operated. Thirty of them were operated with 5/6 nephrectomy. Group B was pathological control group. Group C were fed Bacillus bifidus and Group D were fed lactobacillus. After 1 week all rats were sacrificed as samples of blood, digestive juice and gastrointestinal mucosa were taken. Bacteria on the gastrointestinal mucosa were counted. The concentration of UN, Cr, and UA of blood and digestive juice was determined. Results The number of bacteria on the gastrointestinal mucosa of Group B was less than that of Group A （ P 〈 0.05 ） , but that of Group C and D was more than that of Group A and B. The bacteria number on the gastric mucosa was least and that on the descending colon was most. There was significant difference in the bacteria number and concentration of Cr, UN, and UA in various sites of the gastrointestinal tract （ P 〈 0.05 ）. The concentrations of Cr, UN, and UA in the digestive juice of various sites and serum in Group C and D were lower than those in Group B （ P 〈 0.05 ）. Bacteria planting number in the digestive tract has obvious negative correlation with the concentration of Cr, UN, and UA in the blood and digestive tract. Conclusion Field planting of lactobacillns and Bacillus bifidus, and the concentration of low molecule urotoxin are different in various sites of the gastrointestinal tract. It can decrease the concentration of BUN, Cr, and UA in rats with renal failure by feeding lactobacillus and Bacillus bifidus.

关 键 词：5/6肾切除 双歧杆菌 乳酸杆菌 定植 

分 类 号：R371[医药卫生—病原生物学] R692.5[医药卫生—基础医学]