稽留流产患者绒毛滋养细胞AAH的表达  

Expression of aspartyl-(asparaginyl)beta-hydroxylase in villi in patients with missed abortion

作　　者：米春梅[1] 周昌菊[1] 薛敏[1] 

机构地区：[1]中南大学湘雅三医院妇产科,长沙410013

出　　处：《中南大学学报（医学版）》2009年第1期45-48,共4页Journal of Central South University ：Medical Science

基　　金：湖南省科学技术厅科技计划项目(2007WK3038)~~

摘　　要：目的:研究天冬氨酰-(天冬酰胺酰)β-羟化酶(AAH)在稽留流产患者及正常早孕妇女绒毛组织中的表达定位及差异。方法:选取经B超及β-HCG动态监测证实为稽留流产患者50例作为研究组,其中有已知可能原因者20例为研究组1,未知原因者30例为研究组2。选取同期经B超证实胚胎存活,在门诊要求行人工流产的早孕妇女20例作为对照组。根据稽留宫内时间长短不同将研究组分为≤4周组和>4周组。应用免疫组织化学方法比较各组绒毛滋养细胞中AAH的表达。结果:AAH在稽留流产患者及正常早孕妇女绒毛组织滋养细胞胞浆及胞核表达,稽留流产患者绒毛滋养细胞中AAH表达明显低于正常早孕妇女(P<0.05)。有已知原因及未知原因稽留流产患者之间AAH表达无显著差异(P>0.05)。稽留流产患者胚胎稽留宫内时间长短不同,AAH表达亦无显著差异(P>0.05)。结论:AAH在滋养细胞胞浆及胞核中的低表达可能导致稽留流产的发生。Objective To determine the difference in aspartyl-（ asparaginyl ） beta-hydroxylase （AAH） expression level in villi between patients with missed abortion and normal women with early pregnancy, and to confirm the expression loci of AAH in villi. Methods A total of 50 patients of missed abortion were collected and categorized into a test group, which was subdivided into Group 1 and Group 2. Patients in Group 1 （n = 20 ） were of confirmed etiological disorders while those in Group 2 （ n = 30 ） showed no obviously etiological clues. In addition, 20 women of early pregnancy with artificial abortion were categorized into a control group, whose embryos were sonographically confirmed alive before surgery. The 50 patients of missed abortion were also subdivided into a group within 4 weeks and a group over 4 weeks according to the time that the embryo stayed in utrine after death. Immunohistochemical technique and computer image analysis were used to detect the expression loci and the level of AAH in villi. Results AAH was expressed in the endochylema and nucleus of trephocyte both in missed abortion and normal early pregnancy. The expression level of AAH in villi of missed abortion was much lower than that of in villi of normal early pregnancy （ P 〈 0.05 ）. The expression level had no difference between different groups of patients with missed abortion （P 〉 0. 05 ）. Conclusion Low expression of AAH in the endochylema and nucleus of trephocyte may play a role in patients with missed abortion.

关 键 词：天冬氨酰-(天冬酰胺酰)β-羟化酶 稽留流产 绒毛 

分 类 号：R714.21[医药卫生—妇产科学]