轮状病毒非结构蛋白1与种属限制性的相关性研究  

Correlation between rotavirus NSP1 and species restriction:a bioinformation study

作　　者：刘凯军[1] 李晋涛[1] 魏昆[1] 连冠[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038

出　　处：《第三军医大学学报》2009年第3期194-197,共4页Journal of Third Military Medical University

基　　金："十一五"国家科技支撑计划(2006BAI03B12);国家自然科学基金(30571708);第三军医大学学员创新基金(SYDJCB200702)~~

摘　　要：目的研究轮状病毒(rotavirus,RV)非结构蛋白1(nonstructural protein1,NSP1)与种属限制性的相关性,探讨NSP1在RV种属限制性机制中的作用。方法对UniProt数据库中的RVNSP1C末端、全序列以及GenBank数据库中NSP1基因的3′端非编码序列利用DNAStar(Lasergene)7.1软件进行种属间和种属内的序列比对和进化树分析。结果NSP1种属间的多样性与RV宿主特异性一致,NSP1的C末端、NSP1基因3′端非编码序列与NSP1全序列种属内的一致性较高,且高于种属间一致性(P<0.01)。结论NSP1与RV种属限制性密切相关。NSP1与宿主特异的干扰素调节因子3(interferon regulatory factor3,IRF3)相结合的特性和RV的种属限制性相关,其结合区域可能是决定RV种属限制性的关键区域。NSP1基因3′端非编码序列可能在基因水平参与调控RV种属限制性。Objective To study the correlation between rotavirus nonstructural protein 1 （ NSP1 ） and species restriction of the virus and explore the role of NSP1 in the species restriction. Methods NSP1 C-terminal sequences, its whole amino acid sequences from UniProt Database, and its 3′ noncoding sequences from Gen- Bank were collected, and intraspecies and interspecies sequence alignment and phylogenetic tree analysis were performed by DNAStar （Lasergene）7.1. Results The diversity of NSP1 between species and rotavirus species restriction hosted a high degree of consistency. NSP1 C-terminal sequence, NSP1 gene 3′ noncoding sequence and NSP1 whole sequence shared a high intraspecies identity, which was higher than the identity between species （P 〈0. 01）. Conclusion NSP1 and rotavirus species restriction are closely related. Interaction between NSP1 and host specificity interferon regulatory factor 3 （IRF3） may be related to the rotavirus species restriction, and their binding region and the 3′ noncoding sequence of NSP1 gene may be also related to RV species restriction.

关 键 词：轮状病毒 非结构蛋白1 种属限制性 干扰素调节因子 

分 类 号：Q939.41[生物学—微生物学] R373.2[医药卫生—病原生物学]