sTRAIL真核表达质粒对人胃癌裸鼠移植瘤的抑制作用  

Anti-tumor effect of sTRAIL eukaryotic expressing plasmid on human gastric cancer xenografts in nude mice

作　　者：李彦[1] 姜政[1] 向廷秀[2] 曾波[1] 陶小红[1] 王丕龙[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016 [2]重庆医科大学附属第一医院实验研究中心,重庆400016

出　　处：《第三军医大学学报》2009年第3期198-201,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30500234)~~

摘　　要：目的探讨可溶性肿瘤坏死因子相关凋亡诱导配体(soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL)真核表达质粒对人胃癌裸鼠移植瘤的抑制作用。方法建立胃癌细胞株BGC-823裸鼠移植瘤模型,成瘤后于瘤内多点注射重组真核表达质粒pBud-EGFP-TRAIL,并设pBud-EGFP组和生理盐水组为对照,观察并记录肿瘤的生长情况。Western blot和RT-PCR检测sTRAIL mRNA和蛋白表达情况,HE染色观察肿瘤组织病理改变,TUNEL法及电镜技术观测肿瘤组织细胞凋亡情况。结果注射pBud-EGFP-TRAIL组的sTRAIL mRNA和蛋白为阳性,其瘤体大小明显小于pBud-EGFP组和生理盐水组,差异有统计学意义(P<0.05)。HE染色示pBud-EGFP-TRAIL组肿瘤细胞大量死亡,电镜下可见到典型凋亡细胞,TUNEL法显示其凋亡细胞明显增多。结论sTRAIL真核表达质粒pBud-EGFP-TRAIL瘤内注射对人胃癌裸鼠移植瘤有抑制作用。Objective To study the anti-tumor effect of eukaryotic expressing plasmid encoding soluble tumor necrosis factor-related apoptosis inducing ligand （sTRAIL） on human gastric cancer xenografts in nude mice. Methods Human gastric cancer cell line BGC-823 was inoculated subcutaneously into the back of nude mice and grew to tumor nodules. Multi-point intratumoral injection of pBud-EGFP-TRAIL was performed. The tumor-bearing mice receiving empty plasmid or normal saline served as control. The growth of tumors was observed and recorded. RT-PCR and Western blot analysis were used to detect the expression of sTRAIL in the tumor cells. HE staining was used to see the changes of tumor tissues, and TUNEL and electron microscopy for the apoptosis of cells. Results The protein and mRNA expression of sTRAIL in the pBud-EGFP-TRAIL group were positive, and the volume of tumor was statistically smaller than that of the control groups. Extensive dead tumor cells were seen in the pBud-EGFP-TRAIL group, and remarkable apoptosis was shown by TUNEL and electron microscopy. Conclusion Direct intratumoral injection of sTRAIL eukaryotic expressing plasmid suppresses the growth of gastric cancer xenografts in nude mice.

关 键 词：胃肿瘤 裸鼠 肿瘤坏死因子相关诱导凋亡配体 

分 类 号：R392.12[医药卫生—免疫学] R730.23[医药卫生—基础医学]