Isolation and identification of moderately thermophilic acidophilic iron-oxidizing bacterium and its bioleaching characterization  

作　　者：曾伟民 邬长斌 张汝兵 胡培磊 邱冠周 顾帼华 周洪波 

机构地区：[1]School of Minerals Processing and Bioengineering, Central South University [2]Key Laboratory of Biometallurgy, Ministry of Education, Central South University

出　　处：《中国有色金属学会会刊：英文版》2009年第1期222-227,共6页Transactions of Nonferrous Metals Society of China

基　　金：Project(2004CB619204) supported by the National Basic Research Program of China;Project(50621063) supported by the National Natural Science Foundation of China;Project(DYXM-115-02-2-07) supported by China Ocean Mineral Resources Research and Development Association

摘　　要：A moderately thermophilic acidophilic iron-oxidizing bacterium ZW-1 was isolated from Dexing mine, Jiangxi Province, China. The morphological, biochemical and physiological characteristics, 16S rRNA sequence and bioleaching characterization of strain ZW-1 were studied. The optimum growth temperature is 48 ℃, and the optimum initial pH is 1.9. The strain can grow autotrophically by using ferrous iron or elemental sulfur as sole energy sources. The strain is also able to grow heterotrophically by using peptone and yeast extract powder, but not glucose. The cell density of strain ZW-1 can reach up to 1.02×108 /mL with addition of 0.4 g/L peptone. A phylogenetic tree was constructed by comparing with the published 16S rRNA sequences of the relative bacteria species. In the phylogenetic tree, strain ZW-1 is closely relative to Sulfobacilus acidophilus with more than 99% sequence similarity. The results of bioleaching experiments indicate that the strain could oxidize Fe2+ efficiently, and the maximum oxidizing rate is 0.295 g/(L·h). It could tolerate high concentration of Fe3+ and Cu2+ (35 g/L and 25 g/L, respectively). After 20 d, 44.6% of copper is extracted from chalcopyrite by using strain ZW-1 as inocula.A moderately thermophilic acidophilic iron-oxidizing bacterium ZW-1 was isolated from Dexing mine, Jiangxi Province, China. The morphological, biochemical and physiological characteristics, 16S rRNA sequence and bioleaching characterization of strain ZW-1 were studied. The optimum growth temperature is 48 ~C, and the optimum initial pH is 1.9, The strain can grow autotrophically by using ferrous iron or elemental sulfur as sole energy sources. The strain is also able to grow heterotrophically by using peptone and yeast extract powder, but not glucose. The cell density of strain ZW- 1 can reach up to 1.02 × 10^8/mL with addition of 0.4 g/L peptone. A phylogenetic tree was constructed by comparing with the published 16S rRNA sequences of the relative bacteria species, tn the phylogenetic tree, strain ZW-1 is closely relative to SulJobacilus acidophilus with more than 99% sequence similarity. The results of bioleaching experiments indicate that the strain could oxidize Fez＋ efficiently, and the maximum oxidizing rate is 0.295 g/（L.h）. It could tolerate high concentration of Fe3＋and Cu2＋ （35 g/L and 25 g/L, respectively）. After 20 d, 44.6% of copper is extracted from chalcopyrite by using strain ZW-1 as inocula.

关 键 词：不锈钢 电化学性能 研究 金属材料 

分 类 号：TD95[矿业工程—选矿]