中国朝鲜族群体mtDNA编码区3954—4506nt序列多态性  

Mitochondriai DNA coding region 3954-4506 polymorphism in Korean Chinese population

作　　者：张永吉[1] 徐京男 张美花[1] 韩松瑛[1] 

机构地区：[1]延边大学基础医学院法医学教研室,吉林省延吉市133000 [2]延边州公安局刑事科学技术研究所

出　　处：《中华医学遗传学杂志》2009年第1期106-108,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30660201）

摘　　要：目的了解中国朝鲜族群体线粒体DNA（mitochondrial DNA，mtDNA）编码区39544506nt的序列多态性。方法采用PCR产物直接测序方法对198名吉林省延边朝鲜族自治州朝鲜族无关个体进行单倍型分布调查。结果在198名无关个体中，共分出21种单倍型。遗传变异度为0．5906，偶合概率为0．4124。测序结果与Anderson序列比较，共检测出19个变异位点，14个与MITOMAP收录的基因突变相同，5个未见收录。结论mtDNA编码区多态性位点作为mtDNA控制区多态性位点的补充，联合应用可以提高mtDNA的个体识别能力，为mtDNA在中国朝鲜族法医学鉴定提供了基础数据。Objective To investigate the sequence polymorphism of mtDNA coding region encompassing nt3954-4506 in the Korean Chinese population of Yanbian area. Methods Polymerase chain reaction （PCR） and direct sequencing method were used to detect the haplotype distribution of mtDNA coding region in 198 Korean Chinese individuals. Results Twenty-one haplotypes were observed in the 198 unrelated individuals. The genetic diversity was 0. 5906 and the random match probability was 0. 4124. Compared with the Anderson＇s sequence, 19 nucleotide variants were found,of which 14 were previously registered in MITOMAP, and 5 were novel. Conclusion The obtained data suggest that these sequence polymorphisms are valuable genetic markers for personal identification when combined with mtDAN control region investigation, thus could be used as basic data for the forensic application in Korean Chinese population.

关 键 词：线粒体DNA 编码区 单核苷酸多态性 中周朝鲜族人群 

分 类 号：R686[医药卫生—骨科学]