蜂胶黄酮对人脐静脉内皮细胞基质金属蛋白酶9的影响  

Effect of propolis flavone on expression of matrix metalloproteinase-9 in human umbilical vein endothelial cell

作　　者：李东娟[1] 王家富[1] 李伟[1] 

机构地区：[1]泰山医学院病理学教研室,山东省泰安市271000

出　　处：《中国组织工程研究与临床康复》2009年第2期317-319,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:研究表明,基质金属蛋白酶9与动脉粥样硬化斑块的稳定性有关,蜂胶黄酮具有抗动脉粥样硬化的作用。目的:拟证实蜂胶黄酮对人脐静脉内皮细胞基质金属蛋白酶9表达的影响。设计、时间及地点:对比观察。实验于2008-01/08在山东泰山医学院生命科学研究所完成。材料:蜂胶黄酮由泰山医学院生命科学研究所实验室提取;出生1h内新生儿脐带由山东泰安市中心医院提供,产妇知情同意。方法:进行人脐静脉内皮细胞的体外培养和鉴定。按四甲基偶氮唑盐实验筛选的浓度,分别加入25,50,100,200mg/L不同剂量的蜂胶黄酮处理细胞24h,以空白组做对照。主要观察指标:免疫细胞化学染色和ELISA法测定蜂胶黄酮对基质金属蛋白酶9表达的影响。结果:不同剂量的蜂胶黄酮作用人脐静脉内皮细胞24h后均能明显抑制细胞基质金属蛋白酶9的表达,并且随着浓度的升高抑制作用明显增强。结论:蜂胶黄酮能明显降低人脐静脉内皮细胞基质金属蛋白酶9的表达。BACKGROUND： It has been proved that matrix metalloproteinase-9 （MMP-9） has a correlation with stability of atherosclerotic plaque, and propolis flavone plays a role in anti-atherosclerosis. OBJECTIVE： To investigate the effect of propolis flavone on expression of MMP-9 in human umbilical vein endothelial cell （HUVEC）. DESIGN, TIME AND SETTING： The comparative observation was performed at the Institute of Life Science, Taishan Medical University from January to August 2008. MATERIALS： The propolis flavone was extracted by the Institute of Life Science, Taishan Medical University. The neonatal umbilical cord with 1 hour was supplied by Tai＇an Central Hospital, and the informed consent was obtained from the puerperant. METHODS： HUVEC was cultured and identified in vitro, followed by treated with propolis flavoneat with different concentrations for 24 hours. The blank was used as the control group. MAIN OUTCOME MEASURES： The effect of propolis flavone on expression of MMP-9 in HUVEC was detected by cytoimmunochemical staining and ELISA. RESULTS： Propolis flavonecan with all concentrations can restrain the expression of MMP-9 after co-culture with the cells for 24 hours, which showed in a dose-dependent manner. CONCLUSION： The propolis flavone can obvious depress the expression of matrix metalloproteinase-9 in HUVEC.

关 键 词：黄酮 人脐静脉内皮细胞 基质金属蛋白酶9 蜂胶 

分 类 号：R972.6[医药卫生—药品]