两株产生大环内酯Macrolactin S的海洋细菌的筛选及鉴定  

Screening and identification of two Macrolactin S-producing bacteria from the sea

作　　者：韩文菊[1] 卢小玲[1] 许强芝[1] 刘小宇[1] 杨桥[1] 王国飞[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2009年第1期24-27,共4页Academic Journal of Second Military Medical University

基　　金：国家高科技研究发展("863")计划(2006AA09Z416;2006AA09Z425)~~

摘　　要：目的:从中国东海分离筛选抗菌活性微生物,确定活性菌株的分类地位。方法:随机挑选30株本室保存的分离自中国东海的细菌,利用抗大肠杆菌模型进行活性筛选。对活性菌株进行形态特征、生化特征、盐需求测试及16S rDNA序列分析,并将所测得的序列在NCBI数据库进行相似性搜索,选取其中具有代表性的序列以邻接法构建系统进化树,将菌株鉴定到属。结果:筛选出活性菌株F81612和F201721,它们的最适生长盐浓度分别为10%和7.5%,菌株形态特征、生化性质与Bacillus sp.相符,菌株F81612和F201721的16S rDNA序列分别与Bacillus subtilis和Bacillus amyloliquefaciens相似程度最大。结论:利用抗大肠杆菌模型筛选到两株产大环内酯Macrolactin S的细菌,均为中等嗜盐的海洋Bacillus sp.。Objective：To isolate and screen active marine microorganisms from the East China Sea and to identify the phenotypes of the isolated strains.Methods：Thirty strains of bacteria isolated from the East China Sea were selected randomly,and the active strains were screened out by the anti-Escherichia coli model.The active strains were subjected to physiological and biochemical characteristics,salt-aggregation test,and 16S rDNA sequence analysis.Neighbor-joining trees were constructed by comparing the results of 16S rDNA sequences with sequences described in the BLAST server of the National Center for Biotechnology Information（NCBI）,the strains were subsequently identified to genus level.Results：Strains F81612 and F201721 were screened out with the optimum salinities of 10% and 7.5%,respectively.Their morphology and biochemical characteristics were similar to those of Bacillus sp..16S rDNA sequence analysis showed that sequences of F81612 had a higher similarity to those of Bacillus subtilis,F201721 was similar to Bacillus amyloliquefaciens.Conclusion：Two Macrolactin S-producing strains have been screened out by the anti-Escherichia coli model,and they are identified as moderate halophilic Bacillus sp.

关 键 词：Macrolactin S 芽孢杆菌 鉴定 16S RDNA 

分 类 号：R931.77[医药卫生—生药学]