Tim-3中和抗体对哮喘小鼠T淋巴细胞功能的影响  

Effect of Tim-3 Neutralization Antibody on T-Lymphocyte Function in Asthmatic Mice

作　　者：陆小霞[1] 胡维琨[2] 熊盛道[1] 熊维宁[1] 徐国鹏[1] 兰芬[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸内科,武汉市430030 [2]华中科技大学同济医学院附属同济医院眼科,武汉市430030

出　　处：《医学分子生物学杂志》2009年第1期17-20,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.30801260）

摘　　要：目的探讨Tim-3中和抗体对支气管哮喘（简称哮喘）小鼠外周血及支气管肺泡灌洗液（BALF）中T淋巴细胞体：外增殖功能和细胞因子合成功能的影响。方法分别采集实验组和对照组外周血和BALF，并分离出T淋巴细胞进行体外分组培养，各组分别以不同浓度的Tim-3中和抗体进行干预，并在不同的时间进行检测。用MTT法分别测定各组细胞的增殖情况，用酶联免疫吸附试验（ELISA）测定T淋巴细胞白细胞介素4（IL-4）、干扰素γ（IFN-γ）的水平。结果与对照组比较，Tim-3中和抗体对体外培养的实验组小鼠外周血和BALFT淋巴细胞的增殖均有抑制作用，其作用强度（在-定范围内）随剂量的增加和时间的延长而加强（均P〈0．05）；而且对实验组小鼠的抑制作用明显强于对照组小鼠（P〈0．05）；同时实验组小鼠外周血和BALFT淋巴细胞经抗体干预后，IL-4的表达水平较正常组明显下降，IFN-γ明显增加（P〈0．05）。结论 Tim-3中和抗体能抑制实验组小鼠T淋巴细胞的增殖和IL-4的合成，增加IFN-γ的分泌。Tim-3可能作为哮喘治疗新的分子靶点。Objective To investigate the effect of Tim-3 neutralization antibody on proliferation and cytokine synthesis of T lymphocytes of asthmatic mice. Methods Eighteen Kunming mice were randomly divided into two groups. Eight mice were sensitized as asthmatic model group and the others were taken as the normal control group. T lymphocytes were isolated from peripheral blood mononuclear cells and bronchoalveolar lavage fluid （BALF） cells of the mice, and cultured in vitro with Tim-3 neutralization antibody at different concentrations. Proliferation of T lymphocytes was measured by MTT assay. Expression levels of interleukin 4 （IL-4） and interferin （IFN-γ） were detected with enzyme-linked immunosorbent assay （ELISA） . Results Compared with normal control group, Tim-3 neutralization antibody significantly inhibited T lymphocyte proliferation from both peripheral blood and BALF in asthmatic group in vitro （P 〈0. 05） . The effect was enhanced as the increase of concentration .and the prolongation of time. The effect of Tim-3 neutralization on asthmatic group was higher than on control group, with a significant difference of P 〈 0. 05. In either blood or BALF, the IFN-γ level was significantly elevated, whereas the IL-4 level was much lower in asthmatic group compared with normal group. Conclusions Tim-3 neutralization antibody reduces proliferation of T lymphocytes in asthmatic mice, decrease expression level of IL-4, but increase expression level of IFN-γ, indicating that Tim-3 might be a novel molecular target for asthma therapy.

关 键 词：哮喘 TIM-3 抗体 T淋巴细胞 

分 类 号：R392.111[医药卫生—免疫学]