肌钙蛋白 C-肌钙蛋白Ⅰ亲和层析动力学的模型  

Kinetic model of affinity chromatography between troponin C and troponin Ⅰ

作　　者：丛进阳[1] 盖晓东[1] 汪伟国 沈忠耀[1] 

机构地区：[1]清华大学化学工程系

出　　处：《清华大学学报（自然科学版）》1998年第2期99-103,共5页Journal of Tsinghua University(Science and Technology)

基　　金：国家自然科学基金

摘　　要：肌钙蛋白（Ｔｎ）Ｉ有着极强的心肌特异性，心肌肌钙蛋白是唯一存在于心肌细胞中的异构体，可以通过检测患者血液中的心肌ＴｎＩ含量来确诊急性心肌梗塞。根据在钙离子存在下，ＴｎＣ和ＴｎＩ特异结合的特性，可以利用亲和层析提取纯化ＴｎＩ。根据ＴｎＩ从亲和层析柱的穿透曲线，对Ａｒｎｏｌｄ等人提出的孔内扩散模型进行了分析，证明孔内扩散过程不是此亲和层析过程中的限速步骤。在缓冲液体系（ｐＨ８．０）中，提出了包括以一个正向二级、逆向一级来描述ＴｎＣ—ＴｎＩ反应速率方程，并用实验验证了该模型的正确性，从而首次阐明了ＴｎＣ—ＴｎＩ提取纯化体系的动力学机制，揭示了该过程的限速步骤为ＴｎＣ—ＴｎＩ反应过程。In this paper the kinetics for TnC TnI affinity chromatography has been studied. A kinetics model, which contains the rate equation to describe the TnC TnI reaction system has been proposed. This model has been proved to be accurate according to the breakthrough curve data of TnI from affinity chromatography column. Therefore, the kinetic mechanism of affinity chromatography in the system of TnC TnI has been proposed for the first time. It indicates that the rate limiting step is the interaction between TnI and TnC immobilized on Sepharose 4B gel in the experimental conditions. The various parameters of above mentioned affinity chromatography system are determined by pulse analysis technique. These results have laid a foundation for scaling up, optimizing, designing and controlling this affinity chromatography system.

关 键 词：肌钙蛋白I 肌钙蛋白C 亲和层析 心肌梗塞 

分 类 号：R542.220.4[医药卫生—心血管疾病]