基因工程ROSA小鼠血液部分生理生化指标  

The Blood Physiological and Biochemical Parameters in ROSA Mice

作　　者：田小芸[1] 恽时锋[1] 胡玉红[1] 周森妹[1] 董敏[1] 

机构地区：[1]南京军区南京总医院比较医学科,全军实验动物科普与伦理教育基地,南京210002

出　　处：《实验动物科学》2008年第6期6-8,共3页Laboratory Animal Science

摘　　要：目的测定基因工程B6；129-Gt（ROSA）26Sor小鼠血液生理生化正常值。方法采用全自动血细胞分析仪和全自动生化测定仪能分别对5～6周雄性、6～7周雌性ROSA小鼠的血液常规及生化值进行测定。结果ROSA雄雌小鼠血常规比较，中性粒细胞百分率（GR）、中性粒细胞计数（GR#）、平均红细胞血红蛋白量（MCH）之间比较差异显著（P〈0．05），红细胞计数（RBC）、红细胞比积（Ht）、血红蛋白（Hb）、血小板分布宽度（PDW）、平均血小板体积（MPV）之间比较差异非常显著（P〈0．01）；血液生化值雄雌小鼠比较，血肌酐（Cre）间差异显著（P〈0．05），白蛋白（Alb）、总胆固醇（TC）间差异非常显著（P〈0．01），其它指标差异不显著。结论该小鼠的某些生理生化指标值在雄雌间有一定的差异。Objective To determine normal range of the blood routine values and blood biochemical parameters of the genetically engineered mice （B6;129-Gt （ROSA） 26Sor）. Methods Blood routine examination was performed by the automatic hematology analyzer and blood biochemical parameters were determined by the automatic biochemical analyzer in male ROSA mice aged from 5 to 6 weeks and female ROSA mice aged from 6 to 7 weeks. Results The differences in granulocyte count, percentage of granulocyte, and mean corpuscular hemoglobin were significant （ P 〈 0.05 ） and the differences in red-cell count, hematocrit, haemoglobin and platelet distribution width were very significant （P 〈 0.01 ） between female and male ROSA mice. The difference in blood creatinine level was significant （ P 〈 0.05 ） and the differences in albumin and total cholesterol levels were very significant （ P 〈 0.01 ） between female and male ROSA mice. Conclusion There are definite differences in physiological and biochemical parameters between female and male ROSA mice.

关 键 词：基因工程小鼠 血液常规 血液生物化学 

分 类 号：R331.1[医药卫生—人体生理学]