安络化纤丸联合γ-干扰素对血吸虫肝纤维化鼠TIMP-1及TGF-β_1的影响  

Effect of Recombinant Interferon-γ Combined with Anluohuaxianwan on Expression of TIMP-1 and TGF-β_1 mRNA in Hepatic Fibrosis of Mice Infected with Schistosoma japonica

作　　者：黄海燕[1,2] 焦云桃[1] 艾国[1] 黄铁军[1,3] 黄加权[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030 [2]咸宁市咸安区人民医院病理科,湖北咸宁437100 [3]咸宁学院感染科,湖北咸宁437100

出　　处：《中华医院感染学杂志》2009年第1期30-34,共5页Chinese Journal of Nosocomiology

基　　金：湖北省血吸虫病防治项目(XFo6c28)

摘　　要：目的了解安络化纤丸+γ-干扰素(IFN-γ)对实验性血吸虫病肝纤维化指标TIMP-1、TGF-β1表达的影响。方法将36只昆明小鼠随机分为正常对照组,感染对照组及IFN-γ+安络化纤丸组;采用日本血吸虫尾蚴40条/只鼠攻击感染小鼠建立血吸虫性肝纤维化模型,IFN-γ+安络化纤丸联合干预连续8周,观察肝组织纤维化程度及虫卵结节变化;SABC免疫组织化学检测TIMP-1的表达;实时荧光定量法检测TGF-β1mRNA的含量。结果安络化纤丸+IFN-γ组小鼠肝组织虫卵肉芽肿个数较感染对照组少,虫卵肉芽肿平均直径较感染对照组小,两组相比,差异有统计学意义(P<0.05);安络化纤丸+IFN-γ组小鼠肝组织TIMP-1的水平和TGF-β1mRNA的相对量均较感染对照组显著下降,差异有统计学意义(P<0.05)。结论安络化纤丸+IFN-γ组虫卵肉芽肿减小,数目减少,肝纤维化程度减轻可能与其能减少TIMP-1的表达,下调TGF-β1的转录水平有关。OBJECTIVE To explore the effects of recombinant interferon y and Anluohuaxianwan to TIMP-1 and TGF-β1 mRNA in hepatic fibrosis induced by Schistosoma japonicum in mice. METHODS Thirty-six mice randomly divided into three groups： the normal control group, the infectious control group and the treated group Interferon-γ combined with Anluohuaxianwan. Schistosomal liver fibrosis model was established by infection with 40 S. japonicum cercariae. After 8 weeks of treatment, the mice were sacrificed and liver samples were obtained for observing the histopathological changes, the SABC immunohistochemistry and image pro-plus 5.0 were applied to detect the expression of TIMP-1. The real-time PCR was applied to detect the quantity of TGF-β1 mRNA. RESULTS Compared with infected but untreated group, the amount and the size of granuloma and the level of TIMP-1 in treated group decreased （all P〈0.05）. The quantity of TGF-β1 mRNA in treated group were lower than in infected but untreated group （P〈0.05）. CONCLUSIONS Interferon-γ combined with Anluohuaxianwan could obviously grow down the amount and the size of granuloma, diminishing the degree of liver fibrosis. The possible reasons are to markedly inhibit the expression of TIMP-1 and TGF-β1 mRNA.

关 键 词：肝纤维化 血吸虫 安络化纤丸 Γ-干扰素 TIMP-1 TGF-Β1MRNA 

分 类 号：R383.24[医药卫生—医学寄生虫学]