239株非产超广谱β-内酰胺酶大肠埃希菌对β-内酰胺类抗菌药物及美罗培南的耐药性检测  

Resistance to β-Lactam Antibiotics and Meropenem of 239 Non-ESBLs-producing Escherichia coli Isolates

作　　者：王慧[1] 熊自忠[1] 曹鸿霞[1] 魏艳艳[1] 王中新[2] 

机构地区：[1]安徽医科大学第一附属医院感染病科,安徽合肥230022 [2]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《中华医院感染学杂志》2009年第1期106-107,共2页Chinese Journal of Nosocomiology

基　　金：安徽省自然科学基金(050430804);安徽省优秀青年科技基金(08040106815)

摘　　要：目的了解临床分离大肠埃希菌中非产超广谱β-内酰胺酶(ESBLs)菌株对β-内酰胺类抗菌药物及美罗培南的耐药性检测。方法CLSI表型确证试验-纸片增强法检测非产ESBLs大肠埃希菌,琼脂稀释法测定非产ESBLs大肠埃希菌对8种β-内酰胺类抗菌药物及美罗培南的敏感性。结果非产ESBLs大肠埃希菌株对氨苄西林的耐药率高达90.3%,对头孢唑林、头孢呋辛的耐药率>60.0%,对头孢哌酮/舒巴坦、头孢美唑、头孢吡肟的耐药率分别为19.2%、23.8%、31.8%,但对美罗培南非常敏感、耐药率为2.9%。结论非产ESBLs大肠埃希菌对第三代头孢菌素和美罗培南非常敏感,对氨苄西林和第一、二代头孢菌素耐药显著。OBJECTIVE To investigate the resistance to β-lactam antibiotics and meropenem of non-ESBLs- producing Escherichia coli isolates. METHODS Non ESBLs producers of E. coli isolates were detected by CLSI phenotypic confirmatory test, and susceptibility of these isolates to β-lactam antibiotics and meropenem was tested by agar dilution method. RESULTS The resistance rate of these isolates to ampicillin, cefazolin and cefoxime was more than 60% and that to cefoperazone sulbactam, cefmetazole and cefepime was 19.2%, 23.8% and 31.8%, respectively, but most were susceptible to cefotaxime, ceftazidime, and meropenem. CONCLUSIONS The most isolates of non-ESBLs-producing E. coli are resistant to ampicillin and to the first and second generation eephalosporins, but they are susceptible to meropenem and the third generation cephalosporins.

关 键 词：大肠埃希菌 超广谱Β-内酰胺酶 耐药性 

分 类 号：R378.21[医药卫生—病原生物学]