维生素C、E对血管内皮细胞氧化损伤保护作用  

Effects of vitamin C and vitamin E on ultrastructures of vascular endothelial cells with oxidative damage in vitro

作　　者：王璇[1,2] 高玉霞[3] 常红[1] 刘莉[1] 杨海贤[3] 黄国伟[1] 

机构地区：[1]天津医科大学公共卫生学院营养与食品卫生学教研室,天津300070 [2]天津医科大学附属肿瘤医院病理科 [3]天津医科大学总医院心内科

出　　处：《中国公共卫生》2009年第2期193-195,共3页Chinese Journal of Public Health

摘　　要：目的观察维生素C(VC)、维生素E(VE)对体外培养H2O2氧化损伤血管内皮细胞(VEC)超微结构的影响。方法采用体外细胞培养方法,将血管内皮细胞分为正常对照组,H2O2氧化损伤对照组、VC+H2O2组(VC100μmol/L),VE+H2O2组(VE100μmol/L)。分别将VC、VE加入到培养液中,预孵24h,再加入除菌后的H2O2进行损伤,终止培养后收集细胞,进行电镜观察。结果透射电镜观察VEC,可见H2O2损伤对照组细胞超微结构明显异常,细胞表面呈光滑状,丢失特征性的细胞突起及微绒毛;胞质内线粒体损伤严重,形态模糊;粗面内质网明显扩张呈大囊泡状;溶酶体增多;少数细胞严重水肿,核形不规则,核内染色质部分溶解。VC+H2O2和VE+H2O2组血管内皮细胞超微结构仅有轻度损伤,多数细胞与正常对照组细胞相似;大部分细胞表面微绒毛和胞质突起均趋于正常,胞质内细胞器均在正常范围内;细胞核未见异常,核膜完好,核内染色质分布正常;仅有少数细胞粗面内质网有轻度扩张,线粒体结构模糊不清。结论VC、VE可一定程度保护氧化损伤的血管内皮细胞的超微结构。Objective To observe the effect of vitamin C（VC） and vitamin E（VE） on the ultrastructures of vascular endothelial cells（VECs） following oxidative injury in vitro.Methods VECs cultured in vitro were divided into normal control,H2O2 oxidative control,VC（100 μmol/L）＋H2O2,and VE（100 μmol/L）＋H2O2 groups.VECs were injured by H2O2 after they were incubated with different dosages of VC or VE for 24 hours.The morphological changes of VECs in each group were observed with the electronic microscope.Results The ultrastructures of VECs in the H2O2 control group were significantly different from that of the normal control.The cell surfaces were smooth with the losing of the distinctive cell processes and microvilli.The mitochondria in cytoplasm were damaged seriously and had dim appearance.The rough endoplasmic reticula obviously dilated to big hollow vacuoles in shape.The quantities of lysosomes and endocellular pinocytotic vesicles increased.In a few endothelial cells,swollen mitochondria with invisible ridge and irregular nucleus were seen.The chromatin in nuclear was partly dissolved.In VC＋H2O2 and VE＋H2O2 groups,electron microscopic observation revealed that the ultrastructures of VECs were damaged slightly,most of cells in both groups had the same structures as in the normal control.The microvilli and cytoplasmic processes on the cell surface became common.The cellular organs in cytoplasm were normal.The abnormal nucleus were not seen and the nuclear membrane was intact,nuclear chromatin had normal distribution.Only a few cells had slightly dilated rough endoplasmic reticula and unclear mitochondria.Conclusion To some extent,VC,VE can protect the ultrastructures of VECs following oxidative injury.

关 键 词：维生素C 维生素E H2O2 血管内皮细胞 超微结构 

分 类 号：Q564[生物学—生物化学]