青藏铁路沿线鼠疫菌毒力因子检测  

Detection of virulence factors of Y. pestis along Qinghai-Tibet railway

作　　者：张春华[1] 吕景生[1] 赵斌[1] 丛显斌[1] 王忠惠[1] 张市[1] 邵奎东[1] 

机构地区：[1]中国疾病预防控制中心鼠疫布氏菌病预防控制基地,吉林白城137000

出　　处：《中国公共卫生》2009年第2期207-208,共2页Chinese Journal of Public Health

基　　金：国家"十.五"科技攻关项目资助(2004BA718B08)

摘　　要：目的检测青藏铁路沿线鼠疫菌是否具有公认的毒力因子。方法采用反向间接血凝试验检测FraI;利用假结核菌对PstI敏感可产生抑菌环的复盖种子层法检测PstI;采用乏Ca2+时37℃鼠疫菌不能生长观察Vwa,利用色素培养基观察Pgm。结果所检测青藏铁路沿线菌株都具有PstI+、Vwa+、FraI+、Pgnn+4种毒力因子。结论根据其地理位置和宿主判断所检测菌株属于强毒力菌株。Objective To detect virulence factors of Y.pestis of along Qinghai-Tibet railway.Methods Reverse hemagglutination test was used for identification of Fral.The Y.pseudotuberculosis sensitive to PstI and the bacteriostastic ring were used for identification of PstI.The low-calcium medium was used for identification of Vwa and Jackson-Burrows medium was used for identification of Pgm.Results The Y.pestis of along Qinghai-Tibet railway contain PstI^＋,Vwa^＋,FraI^＋,Pgm^＋ in the experiment.Conclusion All strains detected are high virulent strain considering the geographic area and the host.

关 键 词：青藏铁路沿线 鼠疫菌 毒力因子 

分 类 号：R516.8[医药卫生—内科学]