铝对大鼠学习记忆及海马神经细胞凋亡影响  

Effect of aluminum on learning memory and apoptosis of hippocampus cells in rats

作　　者：高丽萍[1] 程书珍[2] 王晓梅[2] 贾丽霞[2] 

机构地区：[1]北京联合大学应用文理学院生物系生物活性物质与功能食品北京市重点实验室,北京100083 [2]河北工程大学医学院

出　　处：《中国公共卫生》2009年第2期214-215,共2页Chinese Journal of Public Health

基　　金：河北省科技攻关项目(06276102D-56)

摘　　要：目的研究三氯化铝对大鼠学习记忆及海马神经细胞凋亡的影响。方法选择雄性SD大鼠,按体重随机分为4组,在基础饲料中添加三氯化铝,Al3+剂量分别为0,11.2,55.9和111.9mg/(kg.bw),连续染毒3个月。采用Y-电迷宫仪检测大鼠学习记忆能力,原位末端标记技术(TUNEL)和流式细胞术检测大鼠海马神经细胞凋亡,原子吸收法检测海马铝含量。结果染毒Al3+111.9mg/(kg.bw)组大鼠逃避正确率为(0.83±0.115)%明显低于对照组(0.95±0.127)%,<0.05);5.9和111.9mg/(kg.bw)组大鼠逃避潜伏期分别为(4.81±1.33),(5.55±1.79)s,对照组(3.45±1.12)s明显延长(P<0.05);染毒组大鼠海马均出现TUNEL阳性细胞,且随剂量增加渐趋明显,各染毒组大鼠海马神经细胞凋亡率及铝含量明显高于对照组(P<0.01),且呈明显剂量-效应关系。结论铝诱导海马神经细胞凋亡是导致大鼠学习记忆障碍的重要机制之一。Objective To study the effects of aluminum trichloride on learning memory and apoptosis of hippocampus cell.Methods Sprague-Dawley rats were randomly divided into four groups based on their boby weight.Aluminum chloride was added into diet at doses of 0,11.2,55.9and 111.9mg/kg·bw for 90 days successively.Learning and memory abilities were observed by Y-maze.The morphological characters and percent of neuronal apoptosis in hippocampus were identified by teminal deoxynuleotidyl transferase-mediated nick-end labeling（TUNEL） and FACS scan.Aluminum was measured by atomic absorption spectroscopy.Results Correct escaping rate of the rats in the dose group of 111.9mg Al^3＋/kg·bw was decreased.And escaping latency of rats exposed to doses of 55.9 and 111.9mg/kg·bw was prolonged significantly as compared with the controls.In hippocampus,TUNEL-positive cell could be detected in every treatment group.As the dosage of AlCl3 increased,TUNEL-positive appeared to be more obvious.The rates of apoptosis and aluminum content in hippocampus in every treatment group were significantly increased with a singificant does-response relationship.Conclusion Apoptosis induced by aluminum in rat hippocampus might be one of the important mechanisms of the damage of learning and memory.

关 键 词：铝 学习记忆 海马 神经细胞凋亡 

分 类 号：R995[医药卫生—毒理学]