烟草中植物甾醇的形态及分布  

State and Distribution of Phytosterols in Tobacco

作　　者：刘少民[1] 周桂园 方力[3] 童红武[1] 闫向阳[1] 刘文慧[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心,合肥市230052 [2]红河烟草(集团)有限责任公司技术中心,云南省652300 [3]云南烟草科学研究院,昆明市650106

出　　处：《烟草科技》2009年第1期29-36,42,共9页Tobacco Science & Technology

摘　　要：采用浓盐酸水解、KOH乙醇溶液皂化、正己烷萃取、双（三甲基硅烷基）三氟乙酰胺（BSTFA）衍生化和气相色谱法测定了不同种类、不同品种、不同产地、不同部位烟叶样品中游离态和结合态菜油甾醇、豆甾醇和β-谷甾醇的含量。结果表明：①各甾醇的检测限在0．10—0．35μg／mL范围，相对标准偏差2．3％～3．3％，回收率87％～99％；（②烟叶中植物甾醇的总含量与其类型、产地、部位等有非常大的关系；③烟叶中植物甾醇的种类分布也受品种的影响，但部位、产地的影响不大；④烟叶中酯态甾醇（SE）和糖苷态植物甾醇（SG＋ASG）的含量可能分别与其高级脂肪酸和单糖含量正相关。The contents of free and combined eampesterol, stigmasterol and β-sitosterol in tobacco leaves of different types, varieties, growing areas and stalk positions were determined by gas chromatography via hydrolysis by concentrated hydrochloric acid, saponification by an alcoholic KOH solution, extraction with hexane, and derivation with bis （trimethylsilyl）trifluoroacetamide （BSTFA）. The results showed that the limits of detection （LODs） of the sterols varied from 0.10 to 0.35 μg/mL and their recoveries ranged from 87% to 99% with the relative standard deviations （RSDs） of 2.3% to 3.3%. The total phytosterol content in tobacco leaf was greatly affected by tobacco type, stalk position and growing area. The distribution of different kinds of phytosterol in tobacco leaf was also influenced by tobacco variety, while the effects of stalk position and growing area were not obvious. The contents of steryl ester （SE） , steryl glycoside and acylated steryl glycoside （SG ＋ ASG） might be positively correlated with the contents of higher fatty acids and monosaccharide in tobacco leaf, respectively.

关 键 词：植物甾醇 气相色谱 烟草 皂化 

分 类 号：TS411.1[农业科学—烟草工业]