从PDGF、MUC5AC表达的变化探讨健胃愈疡颗粒剂对复发胃溃疡大鼠的作用机制(英文)  

Mechanism of effect of Jianweiyuyang Granules on gastric ulcer recurrence of rat and expression of PDGF and MUC5AC in healing process of rat gastric ulcer

作　　者：戴幸平[1] 刘昭前[2] 蒋荣鑫[1] 周兵 李家邦[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410078 [2]中南大学湘雅医学院临床药理研究所,湖南长沙410006 [3]湖南中医药研究院附属医院,湖南长沙410006

出　　处：《中国现代医学杂志》2009年第1期1-6,共6页China Journal of Modern Medicine

基　　金：Supported by Foundation of Traditional Chinese Medicine supported with government sanitation agency of Hunan province(No.202053)

摘　　要：目的观察健胃愈疡颗粒(jianweiyuyang granules,JWYY)对复发胃溃疡大鼠胃粘膜组织中PDGF、MUC5AC表达的影响及抗复发机制研究。方法腹腔注射IL-1β制作胃溃疡复发模型。运用RT-PCR、Western blotting方法检测胃溃疡复发大鼠PDGF、胃黏膜黏蛋白5AC(MUC5AC)表达的变化。结果健胃愈疡颗粒能明显升高PDGF、MUC5AC的表达水平,且模型组PDGF、MUC5AC的表达水平明显高于对照组(P<0.01)。结论健胃愈疡颗粒通过增高PDGF、MUC5AC的表达,促进血管生成,增加粘膜粘液凝胶厚度,提高溃疡愈合质量,降低IL-1β所致的大鼠胃溃疡复发率,这是JWYY抗消化性溃疡复发的机制之一。[Objective]To study the effect of Jianweiyuyang （JWYY） Granules on gastric ulcer recurrence of rats and investigate the mechanism of JWYY to prevent the recurrence of gastric ulcer. [Methods] The gastric ulcer was induced in rat by Okeba＇s method with some modification and the recurrence model caused by IL-1β. The expression of platelet derived growth factor （PDGF） and MUCSAC were examined by reverse transcription polymerase chain reaction （RT-PCR） and Western blotting in gastric ulcer. [Result] PDGF and MUC5AC expression in gastric tissues during the healing process in JWYY group was all significantly increased compared with other groups （P 〈0.01）. [Conclusion] It is indicated that Jianweiyuyang Granules can increase thickness of mucosal mucus gel layer and stimulate the angiogenesis through increasing the expression of PDGF and MUC5AC and that may be the mechanism of JWYY accelerating ulcer healing and preventing recurrence of gastric ulcer.

关 键 词：健胃愈疡颗粒 胃溃疡复发 PDGF MUC5AC 

分 类 号：R285.5[医药卫生—中药学]