Sca1^+间充质干细胞支持造血干细胞向树突细胞分化  

Sca1^+ mesenchymal stem cells induce hemopoietic stem cells to differentiate into dendritic cells

作　　者：陈媛[1] 刘星霞[1] 赵春华[1] 

机构地区：[1]中国医学科学院基础医学研究所组织工程中心,北京100005

出　　处：《基础医学与临床》2009年第1期10-13,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30570771)

摘　　要：目的研究Sca1+间充质干细胞对HSC向树突细胞(DC)分化的支持作用,并对获得的DC进行形态、表面标志以及功能的鉴定。方法取健康Balb/c小鼠骨髓,通过MACS系统分离、纯化CD117+造血干细胞;以小鼠Sca1+间充质干细胞做饲养层,诱导CD117+造血干细胞定向分化为树突细胞,显微镜观测形态、流式细胞仪检测表面标志,并通过激光共聚焦显微镜和动物实验检测诱生细胞的功能。结果培养第10天诱生的DC细胞表面树突较短小,呈毛刺状,高表达CD11b,低表达Ia,对外源颗粒具有吞噬功能,在动物器官移植实验中具有免疫抑制功能。结论Sca1+间充质干细胞对CD117+HSC向DC分化具有支持作用。Objective To study the effect of Scal ^＋ cells on the HSCs＇（ hemopoietic stem cells） differentiation to dendritic cells （DCs）, and identify the morphology, function and surface markers of the cells. Methods CD117^＋ HSCs were isolated and purified from the bone marrow of healthy Balb/c mice by magnetic affinity cell sorting. After cell expansion by treatment with the support of Scal ^＋ cells, the HSCs were induced for directional differentiation into DC-like cells. Studied the surface markers by FACS and function via LSCM and animal experiments. Results After 10 days of culture, we demonstrated that Scal ^＋ cells induced HSCs to differentiate into a distinct regulatory DC subset with high expression of CD11 b but low expression of Ia. They had phagocytotic activity, and suppressed the GVHD（ graft versus host disease） reaction. Conclusion HSCs can differente into dendritic cells with the support of Scal ^＋ cells.

关 键 词：造血干细胞 树突细胞 定向分化 Sca1^+ 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]