红腹锦鸡胃肠道内分泌细胞的免疫组织化学定位  

Immunohistochemical Localization of Endocrine Cells in the Digestive Tract of Chrysolophus pictus

作　　者：李丽霞[1] 安书成[2] 

机构地区：[1]陕西理工学院生物科学与工程学院,汉中723000 [2]陕西师范大学生命科学学院,西安710062

出　　处：《动物学杂志》2009年第1期24-32,共9页Chinese Journal of Zoology

基　　金：陕西理工学院专项科研基金项目(No.SLG0520)

摘　　要：应用ABC免疫组织化学方法,对红腹锦鸡(Chrysolophus pictus)胃肠道5-羟色胺(5-hydroxtryptamine,5-HT)、胃泌素(gastrin,GAS)、生长抑素(somatostatin,SS)3种内分泌细胞的分布密度和形态进行了观察。结果显示,5-HT细胞在空肠和直肠分布密度最高,回肠和盲肠次之,十二指肠较少,腺胃和肌胃最少;GAS细胞在十二指肠和直肠分布密度最高,其次是空肠和盲肠,腺胃部最低,肌胃则呈免疫阴性;SS细胞数量较少,在直肠、盲肠处分布密度相对高,其次是十二指肠和空肠,腺胃部最低,肌胃则呈免疫阴性。3种内分泌细胞的形态多样,有圆形、椭圆形、锥体形、杆状和不规则形,其中以圆形、椭圆形为主。细胞分布于固有膜、黏膜上皮细胞基部、黏膜上皮细胞之间、腺泡上皮细胞基部或腺泡上皮细胞之间。红腹锦鸡胃肠道内分泌细胞的形态与其内、外分泌功能是相适应的。We investigated the distribution patterns and morphologies of 5-hydroxytryptamine （5-HT）-, gastfin （GAS）- and somatostatin （SS）-endocrine cells in the gastrointestinal tract of Chrysolophus pictus using the ABC immunostaining technique. The 5-HT cells were mostly abundantly distributed in the jejunum and rectum, rich in the ileum and cecum, followed by the duodenum, and rarely in the proventriculus and gizzard tissues; GAS cells were most densely distributed in the duodenum and rectum, followed by the cecum and jejunum, infrequently in the proventriculus tissues, and they were not observed in the stomach muscles; SS ceils were in a low density in the intestine tissues, more concentrated in the rectum and cecum than in the duodenum and jejunum, however, they were not detected in the stomach muscles. Three types of endocrine cells bad a variety of morphologies, such as round, elliptical, cone-shaped, rod-shaped and irregular-shaped, among which the most common shapes were round and cone-shaped. They were mainly located in cell membrane, the basis and interspace of the mucosal epithelial cells and the alveolar epithelial cells as well. In addition, morphologies of these endocrine cells were consistent with their endocrine and exoerine functions.

关 键 词：红腹锦鸡 胃肠道 内分泌细胞 免疫组织化学 

分 类 号：Q954.6[生物学—动物学]