金雕肺的组织结构及KGF、c-Fos和Bax的表达  

Histological Structure and Expression of KGF,c-Fos and Bax in the Lung of Aguila chrysaetos

作　　者：冯红丽[1] 俞诗源[1] 王昱[1] 贾艳芳[1] 邓海平[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《动物学杂志》2009年第1期33-38,共6页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30370163);甘肃自然科学基金项目(No.ZS031-A25-005-Z);甘肃省创新人才基金项目

摘　　要：为了解金雕(Aguila chrysaetos)肺的组织结构特征,观察角质细胞生长因子(KGF)、c-Fos蛋白和Bax蛋白在肺中的表达情况,应用生物显微技术和免疫组织化学方法观察了金雕肺的组织结构,检测KGF、c-Fos和Bax蛋白在肺中的表达。结果表明,金雕的肺由各级支气管及其周围的呼吸毛细管组成,肺房呈辐射状开口于三级支气管周围,呼吸毛细管相互吻合成网状,周围有丰富的毛细血管网。KGF、c-Fos和Bax在三级支气管、肺房和呼吸毛细管的上皮细胞中呈免疫反应阳性,三者的表达强度有差异。KGF、c-Fos和Bax可能发挥不同的功能,它们的协同表达可能调控鸟肺细胞的生长、发育及成熟。Histological structure was observed by light microscopy and expression of KGF, c-Fos and Bax was examined by immunohistochemical methods in Aguila chrysaetos lung. Results showed that the lung was consisted of bronchia and many breathing capillaries （minute trachea） around bronchia; pulmonary atrium radially disposed around tertiary bronchia; many breathing capillaries anastomosed reticulation with each other; and rich pulmonary capillaries distributed around minute trachea. KGF, c-Fos and Bax were expressed in the epithelial ceils of the tertiary bronchia, pulmonary atrium and breathin capillaries, but their expression intensities were different. KGF, c-Fos and Bax may have different functions, and their cooperative expression may control the growth, development and maturity of cells in bird lung.

关 键 词：金雕 肺 组织结构 免疫组织化学 角质细胞生长因子 c—Fos蛋白 BAX蛋白 

分 类 号：Q954.6[生物学—动物学]